| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
MUSCLE CELL BIOLOGY AND CELL MOTILITY
Department of Biological Sciences, Marquette University, Milwaukee Wisconsin 53201
Submitted 23 January 2004 ; accepted in final form 22 April 2004
Elevated levels of Pi are thought to cause a substantial proportion of the loss in muscular force and power output during fatigue from intense contractile activity. However, support for this hypothesis is based, in part, on data from skinned single fibers obtained at low temperatures (
15°C). The effect of high (30 mM) Pi concentration on the contractile function of chemically skinned single fibers was examined at both low (15°C) and high (30°C) temperatures using fibers isolated from rat soleus (type I fibers) and gastrocnemius (type II fibers) muscles. Elevating Pi from 0 to 30 mM at saturating free Ca2+ levels depressed maximum isometric force (Po) by 54% at 15°C and by 19% at 30°C (P < 0.05; significant interaction) in type I fibers. Similarly, the Po of type II fibers was significantly more sensitive to high levels of Pi at the lower (50% decrease) vs. higher temperature (5% decrease). The maximal shortening velocity of both type I and type II fibers was not significantly affected by elevated Pi at either temperature. However, peak fiber power was depressed by 49% at 15°C but by only 16% at 30°C in type I fibers. Similarly, in type II fibers, peak power was depressed by 40 and 18% at 15 and 30°C, respectively. These data suggest that near physiological temperatures and at saturating levels of intracellular Ca2+, elevated levels of Pi contribute less to fatigue than might be inferred from data obtained at lower temperatures.
skinned single fiber; force; power
This article has been cited by other articles:
|
|
 |
|
  S. P. Cairns, D. M. Robinson, and D. S. Loiselle Double-sigmoid model for fitting fatigue profiles in mouse fast- and slow-twitch muscle Exp Physiol, July 1, 2008; 93(7): 851 - 862. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Karatzaferi, K. Franks-Skiba, and R. Cooke Inhibition of shortening velocity of skinned skeletal muscle fibers in conditions that mimic fatigue Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2008; 294(3): R948 - R955. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. H. Fitts The cross-bridge cycle and skeletal muscle fatigue J Appl Physiol, February 1, 2008; 104(2): 551 - 558. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. G. Allen, G. D. Lamb, and H. Westerblad Skeletal Muscle Fatigue: Cellular Mechanisms Physiol Rev, January 1, 2008; 88(1): 287 - 332. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. van der Poel and D. G. Stephenson Effects of elevated physiological temperatures on sarcoplasmic reticulum function in mechanically skinned muscle fibers of the rat Am J Physiol Cell Physiol, July 1, 2007; 293(1): C133 - C141. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Jones, C. J. de Ruiter, and A. de Haan Change in contractile properties of human muscle in relationship to the loss of power and slowing of relaxation seen with fatigue J. Physiol., November 1, 2006; 576(3): 913 - 922. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. W. Bundle, C. L. Ernst, M. J. Bellizzi, S. Wright, and P. G. Weyand A metabolic basis for impaired muscle force production and neuromuscular compensation during sprint cycling Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2006; 291(5): R1457 - R1464. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. T. Knuth, H. Dave, J. R. Peters, and R. H. Fitts Low cell pH depresses peak power in rat skeletal muscle fibres at both 30{degrees}C and 15{degrees}C: implications for muscle fatigue J. Physiol., September 15, 2006; 575(3): 887 - 899. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. P. Debold, J. Romatowski, and R. H. Fitts The depressive effect of Pi on the force-pCa relationship in skinned single muscle fibers is temperature dependent Am J Physiol Cell Physiol, April 1, 2006; 290(4): C1041 - C1050. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Hinken and K. S. McDonald {beta}-Myosin heavy chain myocytes are more resistant to changes in power output induced by ischemic conditions Am J Physiol Heart Circ Physiol, February 1, 2006; 290(2): H869 - H877. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Rossi, M. Maffei, R. Bottinelli, and M. Canepari Temperature dependence of speed of actin filaments propelled by slow and fast skeletal myosin isoforms J Appl Physiol, December 1, 2005; 99(6): 2239 - 2245. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. L. Dutka, L. Cole, and G. D. Lamb Calcium phosphate precipitation in the sarcoplasmic reticulum reduces action potential-mediated Ca2+ release in mammalian skeletal muscle Am J Physiol Cell Physiol, December 1, 2005; 289(6): C1502 - C1512. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. E. Kan, T. E. Buse-Pot, R. Peco, D. Isbrandt, A. Heerschap, and A. de Haan Lower force and impaired performance during high-intensity electrical stimulation in skeletal muscle of GAMT-deficient knockout mice Am J Physiol Cell Physiol, July 1, 2005; 289(1): C113 - C119. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. R Moopanar and D. G Allen Reactive oxygen species reduce myofibrillar Ca2+ sensitivity in fatiguing mouse skeletal muscle at 37{degrees}C J. Physiol., April 1, 2005; 564(1): 189 - 199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Pathare, G. A. Walter, J. E. Stevens, Z. Yang, E. Okerke, J. D. Gibbs, J. L. Esterhai, M. T. Scarborough, C. P. Gibbs, H. L. Sweeney, et al. Changes in inorganic phosphate and force production in human skeletal muscle after cast immobilization J Appl Physiol, January 1, 2005; 98(1): 307 - 314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. L Dutka and G. D Lamb Effect of low cytoplasmic [ATP] on excitation-contraction coupling in fast-twitch muscle fibres of the rat J. Physiol., October 15, 2004; 560(2): 451 - 468. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
