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Am J Physiol Cell Physiol 287: C30-C35, 2004. First published February 25, 2004; doi:10.1152/ajpcell.00443.2003
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MEMBRANE TRANSPORTERS, ION CHANNELS, AND PUMPS

Identification and characterization of a Kidd antigen/UT-B urea transporter expressed in human colon

Hideki Inoue,1,* Shelley D. Jackson,1,* Tatyana Vikulina,1 Janet D. Klein,2 Kimio Tomita,3 and Serena M. Bagnasco1

1Departments of Pathology and 2Medicine, Emory University School of Medicine, Atlanta, Georgia 30322; and 3Third Department of Medicine, University of Kumamoto, Kumamoto, Japan

Submitted 14 October 2003 ; accepted in final form 16 February 2004

We have identified a urea transporter from the mucosa of the human colon that has characteristics consistent with a Kidd antigen/UT-B urea transporter. This intestinal urea transporter encodes a 389-amino acid peptide with a sequence identical to that previously reported for the UT-B urea transporter in erythrocytes. Expression of a UT-B 2-kb mRNA transcript and of ~50- and ~98-kDa UT-B proteins is detected in human colonic mucosa by Northern and Western blot analysis. The UT-B protein is localized in the cell membrane and cytoplasm of the superficial intestinal epithelium and in the epithelial cells in the crypts. A 2-kb UT-B mRNA transcript and the UT-B protein were also identified in the intestinal cell line Caco-2. The transepithelial flux of 14C urea was examined in Caco-2 cells growing on porous membrane support and was significantly inhibited by phloretin, 1,3-dimethylurea, and thiourea, suggesting that the transfer of urea across the Caco-2 monolayer could be mediated, at least in part, by the UT-B urea transporter. We conclude that the Kidd antigen/UT-B urea transporter is physiologically expressed in the human colon epithelium, where it could participate in the transport of urea across the colon mucosa.

urea transport; Caco-2


Address for reprint requests and other correspondence: S. M. Bagnasco, Dept. of Pathology, Johns Hopkins Univ., 600 N. Wolfe St., Baltimore, MD 21287 (E-mail: sbagnas1{at}jhmi.edu).




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