Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 286: C876-C892, 2004. First published December 10, 2003; doi:10.1152/ajpcell.00500.2003
0363-6143/04 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
286/4/C876    most recent
00500.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (5)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hafezi-Moghadam, A.
Right arrow Articles by Cornelssen, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hafezi-Moghadam, A.
Right arrow Articles by Cornelssen, C.

METHODS IN CELL PHYSIOLOGY

A novel mouse-driven ex vivo flow chamber for the study of leukocyte and platelet function

Ali Hafezi-Moghadam,1,2 Kennard L. Thomas,2 and Christian Cornelssen3

1Harvard Medical School and 2Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts 02114; and 3Institute of Mechanics, Faculty V, Technical University, 10623 Berlin, Germany

Submitted 12 November 2003 ; accepted in final form 26 November 2003

Various in vitro and in vivo techniques exist for study of the microcirculation. Whereas in vivo systems impress with their physiological fidelity, in vitro systems excel in the amount of reduction that can be achieved. Here we introduce the autoperfused ex vivo flow chamber designed to study murine leukocytes and platelets under well-defined hemodynamic conditions. In our model, the murine heart continuously drives the blood flow through the chamber, providing a wide range of physiological shear rates. We used a balance of force approach to quantify the prevailing forces at the chamber walls. Numerical simulations show the flow characteristics in the chamber based on a shear-thinning fluid model. We demonstrate specific rolling of wild-type leukocytes on immobilized P-selectin, abolished by a blocking MAb. When uncoated, the surfaces having a constant shear rate supported individual platelet rolling, whereas on areas showing a rapid drop in shear platelets interacted in previously unreported grapelike conglomerates, suggesting an influence of shear rate on the type of platelet interaction. In summary, the ex vivo chamber amounts to an external vessel connecting the arterial and venous systems of a live mouse. This method combines the strengths of existing in vivo and in vitro systems in the study of leukocyte and platelet function.

autoperfused flow chamber; intravital microscopy; inflammation; thrombus formation


Address for reprint requests and other correspondence: A. Hafezi-Moghadam, Massachusetts Eye and Ear Infirmary, Harvard Medical School, 325 Cambridge St., Boston, MA 02114 (E-mail: Ali_Hafezi-Moghadam{at}meei.harvard.edu).




This article has been cited by other articles:


Home page
 FASEB J.Home page
S. Miyahara, L. Almulki, K. Noda, T. Nakazawa, T. Hisatomi, S. Nakao, K. L. Thomas, A. Schering, S. Zandi, S. Frimmel, et al.
In vivo imaging of endothelial injury in choriocapillaris during endotoxin-induced uveitis
FASEB J, June 1, 2008; 22(6): 1973 - 1980.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2004 by the American Physiological Society.