Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 286: C779-C784, 2004. First published November 26, 2003; doi:10.1152/ajpcell.00398.2003
0363-6143/04 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
286/4/C779    most recent
00398.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (28)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Arenas, I. A.
Right arrow Articles by Davidge, S. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Arenas, I. A.
Right arrow Articles by Davidge, S. T.

VASCULAR BIOLOGY

Angiotensin II-induced MMP-2 release from endothelial cells is mediated by TNF-{alpha}

Ivan A. Arenas,1,2 Yi Xu,1,2 Patricio Lopez-Jaramillo,3,4 and Sandra T. Davidge1,2

1Department of Obstetrics and Gynecology and 2Department of Physiology, Perinatal Research Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2S2; and 3Fundacion Cardiovascular del Oriente and 4Universidad Industrial de Santander, Bucaramanga, Colombia

Submitted 17 September 2003 ; accepted in final form 25 November 2003

Angiotensin II (ANG II) has been etiologically linked to vascular disease; however, its role in the alterations of endothelial function that occur in vascular disorders is not completely understood. Matrix metalloproteinases (MMPs) and proinflammatory cytokines are involved in the pathological remodeling of blood vessels that occurs in vascular disease. In this study we evaluated the effects of ANG II on tumor necrosis factor (TNF)-{alpha} and MMP-2 production in endothelial cells. Human umbilical vein endothelial cells (HUVECs) were stimulated with ANG II (0.1–10 µM) for 24 h, in the presence or absence of antagonists of ANG II type 1 (AT1R) and type 2 (AT2R) receptors, and the production and release of TNF-{alpha} and MMP-2 were assessed. ANG II increased TNF-{alpha} mRNA and protein expression and the release of bioactive TNF-{alpha}. Moreover, ANG II induced MMP-2 release and reduced the secretion of tissue inhibitor of MMP (TIMP)-2 from endothelial cells. To elucidate whether endogenous TNF-{alpha} could mediate the effects of ANG II on MMP-2 release, cells were pretreated with anti-TNF-{alpha} neutralizing antibodies or pentoxifylline (an inhibitor of TNF-{alpha} synthesis). TNF-{alpha} inhibition prevented the secretion of MMP-2 induced by ANG II. Furthermore, AT1R antagonism with candesartan prevented the formation of MMP-2 and TNF-{alpha} and the reduction of TIMP-2 induced by ANG II. These results indicate that ANG II, via AT1R, modulates the secretion of TNF-{alpha} and MMP-2 from endothelial cells and that TNF-{alpha} mediates the effects of ANG II on MMP-2 release.

remodeling; vasoactive mediators; inflammation


Address for reprint requests and other correspondence: S. T. Davidge, Perinatal Research Centre, 220 HMRC, Univ. of Alberta, Edmonton, AB, Canada T6G 2S2 (E-mail: sandra.davidge{at}ualberta.ca).




This article has been cited by other articles:


Home page
 HypertensionHome page
S. Sriramula, M. Haque, D. S.A. Majid, and J. Francis
Involvement of Tumor Necrosis Factor-{alpha} in Angiotensin II-Mediated Effects on Salt Appetite, Hypertension, and Cardiac Hypertrophy
Hypertension, May 1, 2008; 51(5): 1345 - 1351.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
M. Flamant, S. Placier, C. Dubroca, B. Esposito, I. Lopes, C. Chatziantoniou, A. Tedgui, J.-C. Dussaule, and S. Lehoux
Role of Matrix Metalloproteinases in Early Hypertensive Vascular Remodeling
Hypertension, July 1, 2007; 50(1): 212 - 218.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
P. K. Mehta and K. K. Griendling
Angiotensin II cell signaling: physiological and pathological effects in the cardiovascular system
Am J Physiol Cell Physiol, January 1, 2007; 292(1): C82 - C97.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. A. Alfonso-Jaume, M. R. Bergman, R. Mahimkar, S. Cheng, Z. Q. Jin, J. S. Karliner, and D. H. Lovett
Cardiac ischemia-reperfusion injury induces matrix metalloproteinase-2 expression through the AP-1 components FosB and JunB
Am J Physiol Heart Circ Physiol, October 1, 2006; 291(4): H1838 - H1846.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
S. Y. Han, Y. H. Jee, K. H. Han, Y. S. Kang, H. K. Kim, J. Y. Han, Y. S. Kim, and D. R. Cha
An imbalance between matrix metalloproteinase-2 and tissue inhibitor of matrix metalloproteinase-2 contributes to the development of early diabetic nephropathy
Nephrol. Dial. Transplant., September 1, 2006; 21(9): 2406 - 2416.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
I. A. Arenas, S. J. Armstrong, Y. Xu, and S. T. Davidge
Tumor Necrosis Factor-{alpha} and Vascular Angiotensin II in Estrogen-Deficient Rats
Hypertension, September 1, 2006; 48(3): 497 - 503.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Y. Ben-Yosef, A. Miller, S. Shapiro, and N. Lahat
Hypoxia of endothelial cells leads to MMP-2-dependent survival and death
Am J Physiol Cell Physiol, November 1, 2005; 289(5): C1321 - C1331.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2004 by the American Physiological Society.