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Am J Physiol Cell Physiol 286: C596-C600, 2004; doi:10.1152/ajpcell.00359.2003
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RECEPTORS AND SIGNAL TRANSDUCTION

Characterization of G proteins involved in activation of nonselective cation channels and arachidonic acid release by norepinephrine/{alpha}1A-adrenergic receptors

Yoshifumi Kawanabe,1,2 Nobuo Hashimoto,1 and Tomoh Masaki2

1Departments of Neurosurgery and 2Pharmacology, Kyoto University Graduate School of Medicine, Kyoto 606-8507, Japan

Submitted 25 August 2003 ; accepted in final form 29 October 2003

We demonstrated recently that norepinephrine activates Ca2+-permeable nonselective cation channels (NSCCs) in Chinese hamster ovary cells stably expressing {alpha}1A-adrenergic receptors (CHO-{alpha}1A). Moreover, extracellular Ca2+ through NSCCs plays essential roles in norepinephrine-induced arachidonic acid release. The purpose of the present study was to identify the G proteins involved in the activation of NSCCs and arachidonic acid release by norepinephrine. For these purposes, we used U73122, an inhibitor of phospholipase C (PLC), and dominant negative mutants of G12 and G13 (G12G228A and G13G225A, respectively). U73122 failed to inhibit NSCCs activation by norepinephrine. The magnitudes of norepinephrine-induced extracellular Ca2+ influx in CHO-{alpha}1A microinjected with G13G225A were smaller than those in CHO-{alpha}1A. In contrast, the magnitudes of norepinephrine-induced extracellular Ca2+ influx in CHO-{alpha}1A microinjected with G12G228A were similar to those in CHO-{alpha}1A. In addition, neither a Rho-associated kinase (ROCK) inhibitor nor a phosphoinositide 3-kinase inhibitor affected norepinephrine-induced extracellular Ca2+ influx. G13G225A, but not G12G228A, also inhibited arachidonic acid release partially. These results demonstrate that 1) the Gq/PLC-pathway is not involved in NSCCs activation by norepinephrine, 2) G13 couples with CHO-{alpha}1A and plays important roles for norepinephrine-induced NSCCs activation, 3) neither ROCK- nor PI3K-dependent cascade is involved in NSCCs activation, and 4) G13 is involved in norepinephrine-induced arachidonic acid release in CHO-{alpha}1A.

norepinephrine; {alpha}1A-adrenergic receptor; nonselective cation channel; G13 protein; arachidonic acid release


Address for reprint requests and other correspondence: Y. Kawanabe, Renal Division, Dept. of Medicine, Brigham and Women's Hospital and Harvard Medical School, Harvard Institute of Medicine, Rm. 520, 77 Ave. Louis Pasteur, Boston, MA 02115 (E-mail; ykawanabe{at}rics.bwh.harvard.edu).




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