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MEMBRANE TRANSPORTERS, ION CHANNELS, AND PUMPS

System-specific O₂ sensitivity of the tandem pore domain K⁺ channel TASK-1

Department of Biology, McMaster University, Hamilton, Ontario, Canada L8S 4K1

Submitted 18 September 2003 ; accepted in final form 16 October 2003

Hypoxic inhibition of TASK-1, a tandem pore domain background K⁺ channel, provides a critical link between reduced O₂ levels and physiological responses in various cell types. Here, we examined the expression and O₂ sensitivity of TASK-1 in immortalized adrenomedullary chromaffin (MAH) cells. In physiological (asymmetrical) K⁺ solutions, 3 µM anandamide or 300 µM Zn²⁺ inhibited a strongly pH-sensitive current. Under symmetrical K⁺ conditions, the anandamide- and Zn²⁺-sensitive K⁺ currents were voltage independent. These data demonstrate the functional expression of TASK-1, and cellular expression of this channel was confirmed by RT-PCR and Western blotting. At concentrations that selectively inhibit TASK-1, anandamide and Zn²⁺ were without effect on the magnitude of the O₂-sensitive current or the hypoxic depolarization. Thus TASK-1 does not contribute to O₂ sensing in MAH cells, demonstrating the failure of a known O₂-sensitive K⁺ channel to respond to hypoxia in an O₂-sensing cell. These data demonstrate that, ultimately, the sensitivity of a particular K⁺ channel to hypoxia is determined by the cell, and we propose that this is achieved by coupling distinct hypoxia signaling systems to individual channels. Importantly, these data also reiterate the indirect O₂ sensitivity of TASK-1, which appears to require the presence of an intracellular mediator.

hypoxia; background K⁺ channels; TASK-1; MAH cells

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