HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 286/1/C153    most recent 00142.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (40) Citing Articles via Google Scholar Google Scholar Articles by Qian, Y. Articles by Jiang, B.-H. Search for Related Content PubMed PubMed Citation Articles by Qian, Y. Articles by Jiang, B.-H.

RECEPTORS AND SIGNAL TRANSDUCTION

PI3K induced actin filament remodeling through Akt and p70S6K1: implication of essential role in cell migration

¹Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown 26506; ²The Mary Babb Randolph Cancer Center and the Department of Microbiology, Immunology, and Cell Biology, West Virginia University, Morgantown, West Virginia 26506-9300; and ³Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115

Submitted 9 April 2003 ; accepted in final form 5 September 2003

This study was designed to identify the molecular mechanisms of phosphatidylinositol 3-kinase (PI3K)-induced actin filament remodeling and cell migration. Expression of active forms of PI3K, v-P3k or Myr-P3k, was sufficient to induce actin filament remodeling to lead to an increase in cell migration, as well as the activation of Akt in chicken embryo fibroblast (CEF) cells. Either the inhibition of PI3K activity using a PI3K-specific inhibitor, LY-294002, or the disruption of Akt activity restored the integrity of actin filaments in CEF cells and inhibited PI3K-induced cell migration. We also found that expression of an activated form of Akt (Myr-Akt) was sufficient to remodel actin filaments to lead to an increase in cell migration, which was unable to be inhibited by the presence of LY-294002. Furthermore, we found that p70S6K1 kinase was a downstream molecule that can mediate the effects of both PI3K and Akt on actin filaments and cell migration. Overexpression of an active form of p70S6K1 was sufficient to induce actin filament remodeling and cell migration in CEF cells, which requires Rac activity. These results demonstrate that activation of PI3K activity alone is sufficient to remodel actin filaments to increase cell migration through the activation of Akt and p70S6K1 in CEF cells.

phosphatidylinositol 3-kinase; Rac; actin filaments

This article has been cited by other articles:

				M. E. Meima, B. A. Webb, H. E. Witkowska, and D. L. Barber The Sodium-Hydrogen Exchanger NHE1 Is an Akt Substrate Necessary for Actin Filament Reorganization by Growth Factors J. Biol. Chem., September 25, 2009; 284(39): 26666 - 26675. [Abstract] [Full Text] [PDF]

				J. A. P. Soares, F. G. G. Leite, L. G. Andrade, A. A. Torres, L. P. De Sousa, L. S. Barcelos, M. M. Teixeira, P. C. P. Ferreira, E. G. Kroon, T. Souto-Padron, et al. Activation of the PI3K/Akt Pathway Early during Vaccinia and Cowpox Virus Infections Is Required for both Host Survival and Viral Replication J. Virol., July 1, 2009; 83(13): 6883 - 6899. [Abstract] [Full Text] [PDF]

				S. Codeluppi, C. I. Svensson, M. P. Hefferan, F. Valencia, M. D. Silldorff, M. Oshiro, M. Marsala, and E. B. Pasquale The Rheb-mTOR Pathway Is Upregulated in Reactive Astrocytes of the Injured Spinal Cord J. Neurosci., January 28, 2009; 29(4): 1093 - 1104. [Abstract] [Full Text] [PDF]

				N. Kakinuma, B. C. Roy, Y. Zhu, Y. Wang, and R. Kiyama Kank regulates RhoA-dependent formation of actin stress fibers and cell migration via 14-3-3 in PI3K-Akt signaling J. Cell Biol., October 14, 2008; 181(3): 537 - 549. [Abstract] [Full Text] [PDF]

				M. Nakhaei-Nejad, A. M. Hussain, Q.-X. Zhang, and A. G. Murray Endothelial PI 3-kinase activity regulates lymphocyte diapedesis Am J Physiol Heart Circ Physiol, December 1, 2007; 293(6): H3608 - H3616. [Abstract] [Full Text] [PDF]

				Q. Zhou, L.-Z. Liu, B. Fu, X. Hu, X. Shi, J. Fang, and B.-H. Jiang Reactive oxygen species regulate insulin-induced VEGF and HIF-1{alpha} expression through the activation of p70S6K1 in human prostate cancer cells Carcinogenesis, January 1, 2007; 28(1): 28 - 37. [Abstract] [Full Text] [PDF]

				K.-S. Chae, K.-S. Oh, and S. E. Dryer Growth Factors Mobilize Multiple Pools of KCa Channels in Developing Parasympathetic Neurons: Role of ADP-Ribosylation Factors and Related Proteins J Neurophysiol, August 1, 2005; 94(2): 1597 - 1605. [Abstract] [Full Text] [PDF]

				G. M. Fuhler, G. J. Knol, A. L. Drayer, and E. Vellenga Impaired interleukin-8- and GRO{alpha}-induced phosphorylation of extracellular signal-regulated kinase result in decreased migration of neutrophils from patients with myelodysplasia J. Leukoc. Biol., February 1, 2005; 77(2): 257 - 266. [Abstract] [Full Text] [PDF]

				Q. Qiu, M. Yang, B. K. Tsang, and A. Gruslin Both mitogen-activated protein kinase and phosphatidylinositol 3-kinase signalling are required in epidermal growth factor-induced human trophoblast migration Mol. Hum. Reprod., September 1, 2004; 10(9): 677 - 684. [Abstract] [Full Text] [PDF]