| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
MUSCLE CELL BIOLOGY AND CELL MOTILITY
Department of Physiological Sciences, Eastern Virginia Medical School, Norfolk, Virginia 23501
Submitted 29 October 2002 ; accepted in final form 22 July 2003
KCl causes smooth muscle contraction by elevating intracellular free Ca2+, whereas receptor stimulation activates an additional mechanism, termed Ca2+ sensitization, that can involve activation of RhoA-associated kinase (ROK) and PKC. However, recent studies support the hypothesis that KCl may also increase Ca2+ sensitivity. Our data showed that the PKC inhibitor GF-109203X did not, whereas the ROK inhibitor Y-27632 did, inhibit KCl-induced tonic (5 min) force and myosin light chain (MLC) phosphorylation in rabbit artery. Y-27632 also inhibited BAY K 8644- and ionomycin-induced MLC phosphorylation and force but did not inhibit KCl-induced Ca2+ entry or peak (
15 s) force. Moreover, KCl and BAY K 8644 nearly doubled the amount of ROK colocalized to caveolae at 30 s, a time that preceded inhibition of force by Y-27632. Colocalization was not inhibited by Y-27632 but was abolished by nifedipine and the calmodulin blocker trifluoperazine. These data support the hypothesis that KCl caused Ca2+ sensitization via ROK activation. We discuss a novel model for ROK activation involving translocation to caveolae that is dependent on Ca2+ entry and involves Ca2+-calmodulin activation.
vascular smooth muscle; signal transduction; caveolin; Y-27632; confocal microscopy
This article has been cited by other articles:
|
|
 |
|
  P. H. Ratz and A. S. Miner Role of Protein Kinase C{zeta} and Calcium Entry in KCl-Induced Vascular Smooth Muscle Calcium Sensitization and Feedback Control of Cellular Calcium Levels J. Pharmacol. Exp. Ther., February 1, 2009; 328(2): 399 - 408. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. R. Kim, C. Gallant, P. C. Leavis, S. J. Gunst, and K. G. Morgan Cytoskeletal remodeling in differentiated vascular smooth muscle is actin isoform dependent and stimulus dependent Am J Physiol Cell Physiol, September 1, 2008; 295(3): C768 - C778. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Villalba, E. Stankevicius, U. Simonsen, and D. Prieto Rho kinase is involved in Ca2+ entry of rat penile small arteries Am J Physiol Heart Circ Physiol, April 1, 2008; 294(4): H1923 - H1932. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Gosens, G. L. Stelmack, G. Dueck, M. M. Mutawe, M. Hinton, K. D. McNeill, A. Paulson, S. Dakshinamurti, W. T. Gerthoffer, J. A. Thliveris, et al. Caveolae facilitate muscarinic receptor-mediated intracellular Ca2+ mobilization and contraction in airway smooth muscle Am J Physiol Lung Cell Mol Physiol, December 1, 2007; 293(6): L1406 - L1418. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. W. Nuno, V. P. Korovkina, S. K. England, and K. G. Lamping RhoA Activation Contributes to Sex Differences in Vascular Contractions Arterioscler Thromb Vasc Biol, September 1, 2007; 27(9): 1934 - 1940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Wardle, M. Gu, Y. Ishida, and R. J. Paul Rho kinase is an effector underlying Ca2+-desensitizing hypoxic relaxation in porcine coronary artery Am J Physiol Heart Circ Physiol, July 1, 2007; 293(1): H23 - H29. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Adebiyi, G. Zhao, S. Y. Cheranov, A. Ahmed, and J. H. Jaggar Caveolin-1 abolishment attenuates the myogenic response in murine cerebral arteries Am J Physiol Heart Circ Physiol, March 1, 2007; 292(3): H1584 - H1592. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hirota, E. Pertens, and L. J. Janssen The reverse mode of the Na+/Ca2+ exchanger provides a source of Ca2+ for store refilling following agonist-induced Ca2+ mobilization Am J Physiol Lung Cell Mol Physiol, February 1, 2007; 292(2): L438 - L447. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Shakirova, J. Bonnevier, S. Albinsson, M. Adner, B. Rippe, J. Broman, A. Arner, and K. Sward Increased Rho activation and PKC-mediated smooth muscle contractility in the absence of caveolin-1. Am J Physiol Cell Physiol, December 1, 2006; 291(6): C1326 - C1335. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. K. Klausen, C. Hougaard, E. K. Hoffmann, and S. F. Pedersen Cholesterol modulates the volume-regulated anion current in Ehrlich-Lettre ascites cells via effects on Rho and F-actin Am J Physiol Cell Physiol, October 1, 2006; 291(4): C757 - C771. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Han, J. E. Speich, T. J. Eddinger, K. M. Berg, A. S. Miner, C. Call, and P. H. Ratz Evidence for absence of latch-bridge formation in muscular saphenous arteries Am J Physiol Heart Circ Physiol, July 1, 2006; 291(1): H138 - H146. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Porter, M. C. Evans, A. S. Miner, K. M. Berg, K. R. Ward, and P. H. Ratz Convergence of Ca2+-desensitizing mechanisms activated by forskolin and phenylephrine pretreatment, but not 8-bromo-cGMP Am J Physiol Cell Physiol, June 1, 2006; 290(6): C1552 - C1559. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. L. Wardle, M. Gu, Y. Ishida, and R. J. Paul Ca2+-desensitizing hypoxic vasorelaxation: pivotal role for the myosin binding subunit of myosin phosphatase (MYPT1) in porcine coronary artery J. Physiol., April 1, 2006; 572(1): 259 - 267. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D. Hardin and J. Vallejo Caveolins in vascular smooth muscle: Form organizing function Cardiovasc Res, March 1, 2006; 69(4): 808 - 815. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Eddinger, J. D. Schiebout, and D. R. Swartz Smooth muscle adherens junctions associated proteins are stable at the cell periphery during relaxation and activation Am J Physiol Cell Physiol, December 1, 2005; 289(6): C1379 - C1387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Liu, J. Zuo, E. Pertens, P. B. Helli, and L. J. Janssen Regulation of Rho/ROCK signaling in airway smooth muscle by membrane potential and [Ca2+]i Am J Physiol Lung Cell Mol Physiol, October 1, 2005; 289(4): L574 - L582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Szaszi, G. Sirokmany, C. D. Ciano-Oliveira, O. D. Rotstein, and A. Kapus Depolarization induces Rho-Rho kinase-mediated myosin light chain phosphorylation in kidney tubular cells Am J Physiol Cell Physiol, September 1, 2005; 289(3): C673 - C685. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Speich, L. Borgsmiller, C. Call, R. Mohr, and P. H. Ratz ROK-induced cross-link formation stiffens passive muscle: reversible strain-induced stress softening in rabbit detrusor Am J Physiol Cell Physiol, July 1, 2005; 289(1): C12 - C21. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D. Smith, E. B. Babiychuk, K. Noble, A. Draeger, and S. Wray Increased cholesterol decreases uterine activity: functional effects of cholesterol alteration in pregnant rat myometrium Am J Physiol Cell Physiol, May 1, 2005; 288(5): C982 - C988. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. H. Ratz, K. M. Berg, N. H. Urban, and A. S. Miner Regulation of smooth muscle calcium sensitivity: KCl as a calcium-sensitizing stimulus Am J Physiol Cell Physiol, April 1, 2005; 288(4): C769 - C783. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Gu, G. D Thorne, R. L Wardle, Y. Ishida, and R. J Paul Ca2+-independent hypoxic vasorelaxation in porcine coronary artery J. Physiol., February 1, 2005; 562(3): 839 - 846. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S Shabir, L Borisova, S. Wray, and T Burdyga Rho-kinase inhibition and electromechanical coupling in rat and guinea-pig ureter smooth muscle: Ca2+-dependent and -independent mechanisms J. Physiol., November 1, 2004; 560(3): 839 - 855. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. T. Sylvester The tone of pulmonary smooth muscle: ROK and Rho music? Am J Physiol Lung Cell Mol Physiol, October 1, 2004; 287(4): L624 - L630. [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. Heaps, J. L. Parker, M. Sturek, and D. K. Bowles Altered calcium sensitivity contributes to enhanced contractility of collateral-dependent coronary arteries J Appl Physiol, July 1, 2004; 97(1): 310 - 316. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
