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PROTEIN AND VESICLE TRAFFICKING, CYTOSKELETON

Potential role for a novel AP180-related protein during endocytosis in MDCK cells

¹Department of Physiology and Biophysics and ²Case Western Reserve University Cancer Center, School of Medicine, Case Western Reserve University, and ³Rainbow Center for Childhood Polycystic Kidney Disease at Rainbow Babies and Children's Hospital of Cleveland, Cleveland, Ohio 44106

Submitted 27 February 2003 ; accepted in final form 17 June 2003

Clathrin assembly protein, AP180, was originally identified as a brain-specific protein localized to the presynaptic junction. AP180 acts to limit vesicle size and maintain a pool of releasable synaptic vesicles during rapid recycling. In this study, we show that polarized epithelial Madin-Darby canine kidney (MDCK) cells express two AP180-related proteins: the ubiquitously expressed 62-kDa clathrin assembly lymphoid myeloid leukemia (CALM, AP180-2) protein and a novel high-molecular-weight homolog that we have named AP180-3. Sequence analysis of AP180-3 expressed in MDCK cells shows high homology to AP180 from rat brain. AP180-3 contains conserved motifs found in brain-specific AP180, including the epsin NH₂-terminal homology (ENTH) domain, the binding site for the -subunit of AP-2, and DLL repeats. Our studies show that AP180-3 from MDCK cells forms complexes with AP-2 and clathrin and that membrane recruitment of these complexes is modulated by phosphorylation. We demonstrate by immunohistochemistry that AP180-3 is localized to cytoplasmic vesicles in MDCK cells and is also present in tubule epithelial cells from mouse kidney. We observed by immunodetection that a high-molecular-weight AP180-related protein is expressed in numerous cells in addition to MDCK cells.

clathrin assembly lympoid myeloid leukemia; kidney epithelial cells; epsin NH₂-terminal homology domain; DLL repeats; clathrin; AP-2

This article has been cited by other articles:

				J. Z. Rappoport, S. Kemal, A. Benmerah, and S. M. Simon Dynamics of clathrin and adaptor proteins during endocytosis Am J Physiol Cell Physiol, November 1, 2006; 291(5): C1072 - C1081. [Abstract] [Full Text] [PDF]