Am J Physiol Cell Physiol AJP: Lung Cellular and Molecular Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 285: C1174-C1187, 2003. First published July 9, 2003; doi:10.1152/ajpcell.00015.2003
0363-6143/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
285/5/C1174    most recent
00015.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (25)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Guo, X.
Right arrow Articles by Wang, J.-Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Guo, X.
Right arrow Articles by Wang, J.-Y.

MEMBRANE TRANSPORTERS, ION CHANNELS, AND PUMPS

Regulation of adherens junctions and epithelial paracellular permeability: a novel function for polyamines

Xin Guo,1,3 Jaladanki N. Rao,1,3 Lan Liu,1,3 Tong-Tong Zou,1,3 Douglas J. Turner,1,3 Barbara L. Bass,1,3 and Jian-Ying Wang1,2,3

Departments of 1Surgery and 2Pathology, University of Maryland School of Medicine, and 3Baltimore Department of Veterans Affairs Medical Center, Baltimore, Maryland 21201

Submitted 13 January 2003 ; accepted in final form 7 July 2003

Maintenance of intestinal mucosal epithelial integrity requires polyamines that are involved in the multiple signaling pathways controlling gene expression and different epithelial cell functions. Integrity of the intestinal epithelial barrier depends on a complex of proteins composing different intercellular junctions, including tight junctions, adherens junctions, and desmosomes. E-cadherin is primarily found at the adherens junctions and plays a critical role in cell-cell adhesions that are fundamental to formation of the intestinal epithelial barrier. The current study determined whether polyamines regulate intestinal epithelial barrier function by altering E-cadherin expression. Depletion of cellular polyamines by {alpha}-difluoromethylornithine (DFMO) reduced intracellular free Ca2+ concentration ([Ca2+]cyt), decreased E-cadherin expression, and increased paracellular permeability in normal intestinal epithelial cells (IEC-6 line). Polyamine depletion did not alter expression of tight junction proteins such as zona occludens (ZO)-1, ZO-2, and junctional adhesion molecule (JAM)-1. Addition of exogenous polyamine spermidine reversed the effects of DFMO on [Ca2+]cyt and E-cadherin expression and restored paracellular permeability to near normal. Elevation of [Ca2+]cyt by the Ca2+ ionophore ionomycin increased E-cadherin expression in polyamine-deficient cells. In contrast, reduction of [Ca2+]cyt by polyamine depletion or removal of extracellular Ca2+ not only inhibited expression of E-cadherin mRNA but also decreased the half-life of E-cadherin protein. These results indicate that polyamines regulate intestinal epithelial paracellular barrier function by altering E-cadherin expression and that polyamines are essential for E-cadherin expression at least partially through [Ca2+]cyt.

ornithine decarboxylase; tight junction; catenins; intracellular calcium; normal intestinal epithelial cells


Address for reprint requests and other correspondence: J.-Y. Wang, Dept. of Surgery, Baltimore Department of Veterans Affairs Medical Center, 10 N. Greene St., Baltimore, MD 21201 (E-mail: jwang{at}smail.umaryland.edu).




This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
L. Khailova, K. Dvorak, K. M. Arganbright, M. D. Halpern, T. Kinouchi, M. Yajima, and B. Dvorak
Bifidobacterium bifidum improves intestinal integrity in a rat model of necrotizing enterocolitis
Am J Physiol Gastrointest Liver Physiol, November 1, 2009; 297(5): G940 - G949.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. Liu, X. Guo, J. N. Rao, T. Zou, L. Xiao, T. Yu, J. A. Timmons, D. J. Turner, and J.-Y. Wang
Polyamines regulate E-cadherin transcription through c-Myc modulating intestinal epithelial barrier function
Am J Physiol Cell Physiol, April 1, 2009; 296(4): C801 - C810.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. Chen, L. Xiao, J. N. Rao, T. Zou, L. Liu, E. Bellavance, M. Gorospe, and J.-Y. Wang
JunD Represses Transcription and Translation of the Tight Junction Protein Zona Occludens-1 Modulating Intestinal Epithelial Barrier Function
Mol. Biol. Cell, September 1, 2008; 19(9): 3701 - 3712.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
B. S. Marasa, L. Xiao, J. N. Rao, T. Zou, L. Liu, J. Wang, E. Bellavance, D. J. Turner, and J.-Y. Wang
Induced TRPC1 expression increases protein phosphatase 2A sensitizing intestinal epithelial cells to apoptosis through inhibition of NF-{kappa}B activation
Am J Physiol Cell Physiol, May 1, 2008; 294(5): C1277 - C1287.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. Chen, J. N. Rao, T. Zou, L. Liu, B. S. Marasa, L. Xiao, X. Zeng, D. J. Turner, and J.-Y. Wang
Polyamines are required for expression of Toll-like receptor 2 modulating intestinal epithelial barrier integrity
Am J Physiol Gastrointest Liver Physiol, September 1, 2007; 293(3): G568 - G576.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A.-H. Zhang, J. N. Rao, T. Zou, L. Liu, B. S. Marasa, L. Xiao, J. Chen, D. J. Turner, and J.-Y. Wang
p53-Dependent NDRG1 expression induces inhibition of intestinal epithelial cell proliferation but not apoptosis after polyamine depletion
Am J Physiol Cell Physiol, July 1, 2007; 293(1): C379 - C389.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
C. Muller, M. Sendler, and J.-P. Hildebrandt
Downregulation of aquaporins 1 and 5 in nasal gland by osmotic stress in ducklings, Anas platyrhynchos: implications for the production of hypertonic fluid
J. Exp. Biol., October 15, 2006; 209(20): 4067 - 4076.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. N. Rao, O. Platoshyn, V. A. Golovina, L. Liu, T. Zou, B. S. Marasa, D. J. Turner, J. X.-J. Yuan, and J.-Y. Wang
TRPC1 functions as a store-operated Ca2+ channel in intestinal epithelial cells and regulates early mucosal restitution after wounding
Am J Physiol Gastrointest Liver Physiol, April 1, 2006; 290(4): G782 - G792.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Pathol.Home page
G A Gill, A Buda, M Moorghen, P W Dettmar, and M Pignatelli
Characterisation of adherens and tight junctional molecules in normal animal larynx; determining a suitable model for studying molecular abnormalities in human laryngopharyngeal reflux
J. Clin. Pathol., December 1, 2005; 58(12): 1265 - 1270.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
T. Zou, J. N. Rao, L. Liu, B. S. Marasa, K. M. Keledjian, A.-H. Zhang, L. Xiao, B. L. Bass, and J.-Y. Wang
Polyamine depletion induces nucleophosmin modulating stability and transcriptional activity of p53 in intestinal epithelial cells
Am J Physiol Cell Physiol, September 1, 2005; 289(3): C686 - C696.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
X. Guo, J. N. Rao, L. Liu, T. Zou, K. M. Keledjian, D. Boneva, B. S. Marasa, and J.-Y. Wang
Polyamines are necessary for synthesis and stability of occludin protein in intestinal epithelial cells
Am J Physiol Gastrointest Liver Physiol, June 1, 2005; 288(6): G1159 - G1169.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. Greenough, L. Pase, I. Voskoboinik, M. J. Petris, A. W. O'Brien, and J. Camakaris
Signals regulating trafficking of Menkes (MNK; ATP7A) copper-translocating P-type ATPase in polarized MDCK cells
Am J Physiol Cell Physiol, November 1, 2004; 287(5): C1463 - C1471.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2003 by the American Physiological Society.