HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 285/5/C1071    most recent 00104.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (17) Citing Articles via Google Scholar Google Scholar Articles by Bertrand, A. Articles by Tuil, D. Search for Related Content PubMed PubMed Citation Articles by Bertrand, A. Articles by Tuil, D.

MUSCLE CELL BIOLOGY AND CELL MOTILITY

Muscle electrotransfer as a tool for studying muscle fiber-specific and nerve-dependent activity of promoters

Institut National de la Santé et de la Recherche Médicale U567, Centre National de la Recherche Scientifique Unité Mixte de Recherche 8104, Institut Cochin, Département Génétique, Développement et Pathologie Moléculaire, Université René Descartes Paris V, 75014 Paris, France

Submitted 19 March 2003 ; accepted in final form 26 June 2003

Muscle electrotransfer has recently become a promising tool for efficient delivery of plasmids and transgene expression in skeletal muscle. This technology has been mainly applied to use of muscle as a bioreactor for production of therapeutic proteins. However, it remains to be determined whether muscle electrotransfer may also be accurately used as an alternative tool to transgenesis for studying aspects of muscle-specific gene control that must be explored in fully mature muscle fibers in vivo, such as fiber specificity and nerve dependence. It was also not known to what extent the initial electrical stimulations alter muscle physiology and gene expression. Therefore, optimized conditions of skeletal muscle electroporation were first tested for their effects on muscles of transgenic mice harboring a pM310-CAT transgene in which the CAT reporter gene was under control of the fast IIB fiber-specific and nerve-dependent aldolase A pM promoter. Surprisingly, electrostimulation led to a drastic but transient shutdown of pM310-CAT transgene expression concomitant with very transient activation of MyoD and, mostly, with activation of myogenin, suggesting profound alterations in transcriptional status of the electroporated muscle. Return to a normal transcriptional state was observed 7-10 days after electroporation. Therefore, we investigated whether a reporter construct placed under control of pM could exhibit fiber-specific expression 10 days after electrotransfer in either fast tibialis anterior or slow soleus muscle. We show that not only fiber specificity, but also nerve dependence, of a pM-driven construct can be reproduced. However, after electrotransfer, pM displayed a less tight control than previously observed for the same promoter when integrated in a chromatin context.

fast or slow fibers; aldolase A; MyoD; myogenin

This article has been cited by other articles:

				H. Tang, P. Macpherson, M. Marvin, E. Meadows, W. H. Klein, X.-J. Yang, and D. Goldman A Histone Deacetylase 4/Myogenin Positive Feedback Loop Coordinates Denervation-dependent Gene Induction and Suppression Mol. Biol. Cell, February 1, 2009; 20(4): 1120 - 1131. [Abstract] [Full Text] [PDF]

				C. R. Benton, Y. Yoshida, J. Lally, X.-X. Han, H. Hatta, and A. Bonen PGC-1{alpha} increases skeletal muscle lactate uptake by increasing the expression of MCT1 but not MCT2 or MCT4 Physiol Genomics, September 17, 2008; 35(1): 45 - 54. [Abstract] [Full Text] [PDF]

				S.-E. Chen, B. Jin, and Y.-P. Li TNF-{alpha} regulates myogenesis and muscle regeneration by activating p38 MAPK Am J Physiol Cell Physiol, May 1, 2007; 292(5): C1660 - C1671. [Abstract] [Full Text] [PDF]

				C. Charvet, C. Houbron, A. Parlakian, J. Giordani, C. Lahoute, A. Bertrand, A. Sotiropoulos, L. Renou, A. Schmitt, J. Melki, et al. New Role for Serum Response Factor in Postnatal Skeletal Muscle Growth and Regeneration via the Interleukin 4 and Insulin-Like Growth Factor 1 Pathways. Mol. Cell. Biol., September 1, 2006; 26(17): 6664 - 6674. [Abstract] [Full Text] [PDF]

				A. Sacco, R. Doyonnas, M. A. LaBarge, M. M. Hammer, P. Kraft, and H. M. Blau IGF-I increases bone marrow contribution to adult skeletal muscle and enhances the fusion of myelomonocytic precursors J. Cell Biol., November 7, 2005; 171(3): 483 - 492. [Abstract] [Full Text] [PDF]

				D. A. Dean Nonviral gene transfer to skeletal, smooth, and cardiac muscle in living animals Am J Physiol Cell Physiol, August 1, 2005; 289(2): C233 - C245. [Abstract] [Full Text] [PDF]

				Z. A Rana, K. Gundersen, A. Buonanno, and D. Vullhorst Imaging transcription in vivo: distinct regulatory effects of fast and slow activity patterns on promoter elements from vertebrate troponin I isoform genes J. Physiol., February 1, 2005; 562(3): 815 - 828. [Abstract] [Full Text] [PDF]

				T. Akimoto, B. S. Sorg, and Z. Yan Real-time imaging of peroxisome proliferator-activated receptor-{gamma} coactivator-1{alpha} promoter activity in skeletal muscles of living mice Am J Physiol Cell Physiol, September 1, 2004; 287(3): C790 - C796. [Abstract] [Full Text] [PDF]

				J. L. Kirby, L. Yang, J. C. Labus, R. J. Lye, N. Hsia, R. Day, G. A. Cornwall, and B. T. Hinton Characterization of Epididymal Epithelial Cell-Specific Gene Promoters by In Vivo Electroporation Biol Reprod, August 1, 2004; 71(2): 613 - 619. [Abstract] [Full Text] [PDF]

				R. Grifone, C. Laclef, F. Spitz, S. Lopez, J. Demignon, J.-E. Guidotti, K. Kawakami, P.-X. Xu, R. Kelly, B. J. Petrof, et al. Six1 and Eya1 Expression Can Reprogram Adult Muscle from the Slow-Twitch Phenotype into the Fast-Twitch Phenotype Mol. Cell. Biol., July 15, 2004; 24(14): 6253 - 6267. [Abstract] [Full Text] [PDF]