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Am J Physiol Cell Physiol 285: C618-C622, 2003. First published May 21, 2003; doi:10.1152/ajpcell.00553.2002
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MEMBRANE TRANSPORTERS, ION CHANNELS, AND PUMPS

The effect of changes in ambient oxygen concentration on the bioelectric properties of middle ear mucosa

David L. Mandell,1,2 Daniel C. Devor,3 Joseph V. Madia,1 Chia-Yee Lo,1 Hilary Hake,1 and Patricia A. Hebda1,2,3

1Department of Pediatric Otolaryngology, Children's Hospital of Pittsburgh, Pittsburgh, Pennsylvania; and Departments of 2Otolaryngology and 3Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, Pennsylvania 15213

Submitted 26 November 2002 ; accepted in final form 7 May 2003

The purpose of the present study was to compare the effect of 24 h of exposure to 7% O2 (normal middle ear physiological conditions) vs. 21% O2 (found in the middle ear after ventilation tube placement) on transepithelial Na+ absorption and Cl– secretion in cultured gerbil middle ear epithelial cell monolayers. Although no difference in apical Na+ absorption was identified, the UTP-induced stimulation of apical Cl– secretion in the presence of apical Na+ channel blockade with amiloride was significantly enhanced after exposure to 21% O2 compared with 7% O2 exposure. In the presence of a calcium-activated Cl– channel inhibitor, DIDS, UTP-induced stimulation of Cl– secretion after 21% O2 exposure was decreased, suggesting a role for calcium-activated Cl– channels in middle ear Cl– secretion in response to relative hyperoxia.

ion channels; sodium; chloride; hypoxia


Address for reprint requests and other correspondence: D. L. Mandell, Dept. of Pediatric Otolaryngology, Children's Hospital of Pittsburgh, Pittsburgh, PA 15213.






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