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Am J Physiol Cell Physiol 285: C608-C617, 2003. First published May 7, 2003; doi:10.1152/ajpcell.00084.2003
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MEMBRANE TRANSPORTERS, ION CHANNELS, AND PUMPS

Identification of an apical exchanger in rat kidney proximal tubule

Snezana Petrovic,1,2 Liyun Ma,2 Zhaohui Wang,1 and Manoocher Soleimani1,2

1Department of Medicine, University of Cincinnati, Cincinnati 45267; and 2Veterans Affairs Medical Center, Cincinnati, Ohio 45220

Submitted 3 March 2003 ; accepted in final form 5 May 2003

SLC26A6 (or putative anion transporter 1, PAT1) is located on the apical membrane of mouse kidney proximal tubule and mediates exchange in in vitro expression systems. We hypothesized that PAT1 along with a exchange is present in apical membranes of rat kidney proximal tubules. Northern hybridizations indicated the exclusive expression of SLC26A6 (PAT1 or CFEX) in rat kidney cortex, and immunocytochemical staining localized SLC26A6 on the apical membrane of proximal tubules, with complete prevention of the labeling with the preadsorbed serum. To examine the functional presence of apical exchanger, proximal tubules were isolated, microperfused, loaded with the pH-sensitive dye BCPCF-AM, and examined by digital ratiometric imaging. The pH of the perfusate and bath was kept at 7.4. Buffering capacity was measured, and transport rates were calculated as equivalent base flux. The results showed that in the presence of basolateral DIDS (to inhibit cotransporter 1) and apical EIPA (to inhibit Na+/H+ exchanger 3), the magnitude of cell acidification in response to addition of luminal Cl– was ~5.0-fold higher in the presence than in the absence of . The Cl–-dependent base transport was inhibited by ~61% in the presence of 0.5 mM luminal DIDS. The presence of physiological concentrations of oxalate in the lumen (200 µM) did not affect the exchange activity. These results are consistent with the presence of SLC26A6 (PAT1) and exchanger activity in the apical membrane of rat kidney proximal tubule. We propose that SLC26A6 is likely responsible for the apical (and Cl–/OH–) exchanger activities in kidney proximal tubule.

putative anion transporter 1; chloride/formate exchanger; SLC26A6


Address for reprint requests and other correspondence: M. Soleimani, Division of Nephrology and Hypertension, Dept. of Medicine, Univ. of Cincinnati, 231 Albert Sabin Way, MSB G259, Cincinnati Medical Center, Cincinnati, OH 45267-0585 (E-mail: Manoocher.Soleimani{at}uc.edu).




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