Am J Physiol Cell Physiol AJP: Regulatory, Integrative and Comparative Physiology
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Am J Physiol Cell Physiol 285: C260-C267, 2003. First published April 9, 2003; doi:10.1152/ajpcell.00560.2002
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MEMBRANE TRANSPORTERS, ION CHANNELS, AND PUMPS

Differential expression and distribution of Kir5.1 and Kir4.1 inwardly rectifying K+ channels in retina

Masaru Ishii,1 Akikazu Fujita,1,2 Kaori Iwai,1 Shunji Kusaka,1,3 Kayoko Higashi,1 Atsushi Inanobe,1 Hiroshi Hibino,1 and Yoshihisa Kurachi1

Departments of 1Pharmacology II and 3Ophthalmology, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871; and 2Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Science, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan

Submitted 2 December 2002 ; accepted in final form 8 April 2003

Kir5.1 is an inwardly rectifying K+ channel subunit whose functional role has not been fully elucidated. Expression and distribution of Kir5.1 in retina were examined with a specific polyclonal antibody. Kir5.1 immunoreactivity was detected in glial Müller cells and in some retinal neurons. In the Kir5.1-positive neurons the expression of glutamic acid decarboxylase (GAD65) was detected, suggesting that they may be GABAergic-amacrine cells. In Müller cells, spots of Kir5.1 immunoreactivity distributed diffusely at the cell body and in the distal portions, where Kir4.1 immunoreactivity largely overlapped. In addition, Kir4.1 immunoreactivity without Kir5.1 was strongly concentrated at the endfoot of Müller cells facing the vitreous surface or in the processes surrounding vessels. The immunoprecipitant obtained from retina with anti-Kir4.1 antibody contained Kir5.1. These results suggest that heterotetrameric Kir4.1/Kir5.1 channels may exist in the cell body and distal portion of Müller cells, whereas homomeric Kir4.1 channels are clustered in the endfeet and surrounding vessels. It is possible that homomeric Kir4.1 and heteromeric Kir4.1/Kir5.1 channels play different functional roles in the K+-buffering action of Müller cells.

inwardly rectifying potassium channel; heteromerization; glial Müller cells; amacrine cells; potassium siphoning


Address for reprint requests and other correspondence: Y. Kurachi, Dept. of Pharmacology II, Graduate School of Medicine, Osaka Univ., Yamada-oka 2-2, Suita, Osaka 565-0871, Japan (E-mail: ykurachi{at}pharma2.med.osaka-u.ac.jp).




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