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Am J Physiol Cell Physiol 285: C112-C118, 2003. First published March 12, 2003; doi:10.1152/ajpcell.00334.2002
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VASCULAR BIOLOGY

High-temporal-resolution analysis demonstrates that ICAM-1 stabilizes WEHI 274.1 monocytic cell rolling on endothelium

Christopher G. Kevil,1 John H. Chidlow,2 Daniel C. Bullard,1 and Dennis F. Kucik3,4

Departments of 1Genomics and Pathobiology and 3Pathology, University of Alabama at Birmingham, Birmingham, Alabama 35294; 2Department of Pathology, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130; and4 Research Service, Birmingham Department of Veterans Affairs Medical Center, Birmingham, Alabama 35233

Submitted 18 July 2002 ; accepted in final form 3 March 2003

Leukocyte rolling, adhesion, and migration on vascular endothelium involve several sets of adhesion molecules that interact simultaneously. Each of these receptor-ligand pairs may play multiple roles. We examined the role of ICAM-1 in adhesive interactions with mouse aortic endothelial cells (MAECs) in an in vitro flow system. Average rolling velocity of the monocytic cell line WEHI 274.1 was increased on ICAM-1-deficient MAECs compared with wild-type MAECs, both with and without TNF-{alpha} stimulation. High-temporal-resolution analysis provided insights into the underlying basis for these differences. Without TNF-{alpha} stimulation, average rolling velocity was slower on wild-type than on ICAM-1-deficient endothelium because of brief (<1 s) pauses. On TNF-{alpha}-stimulated ICAM-1-deficient endothelium, cells rolled faster because of transient accelerations, producing "jerky" rolling. Firm adhesion to ICAM-1-deficient MAECs was significantly reduced compared with wild-type MAECs, although the number of rolling cells was similar. These results demonstrate directly that ICAM-1 affects rolling velocity by stabilizing leukocyte rolling.

intercellular adhesion molecule-1; cell adhesion; leukocytes; vascular endothelium; videomicroscopy


Address for reprint requests and other correspondence: D. F. Kucik, Univ. of Alabama at Birmingham, VHG019 Dept. of Pathology, 1670 University Blvd., Birmingham, AL 35294 (E-mail: kucik{at}uab.edu).




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