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and tumor necrosis factor-
on
gene expression in human endothelial cells
1 Pharmaceutics Division, College of Pharmacy, University of Texas at Austin, Austin 78712; and 2 US Army Institute of Surgical Research and Clinical Investigation, Brook Army Medical Center, San Antonio, Texas 78234
Interleukin-1
(IL-1) and tumor necrosis factor-
(TNF-) are two major
cytokines that rise to relatively high levels during systemic
inflammation, and the endothelial cell (EC) response to these cytokines
may explain some of the dysfunction that occurs. To better understand
the cytokine-induced responses of EC at the gene expression level,
human umbilical vein EC were exposed to IL-1 or TNF- for various
times and subjected to cDNA microarray analyses to study alterations in
their mRNA expression. Of ~4,000 genes on the microarray, expression
levels of 33 and 58 genes appeared to be affected by treatment with
IL-1 and TNF-, respectively; 25 of these genes responded to both
treatments. These results suggest that the effects of IL-1 and
TNF- on EC are redundant and that it may be necessary to suppress
both cytokines simultaneously to ameliorate the systemic response.
recombinant cytokines; microarray analysis; human cells; vascular system
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