Am J Physiol Cell Physiol Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 284: C1561-C1576, 2003. First published January 29, 2003; doi:10.1152/ajpcell.00562.2002
0363-6143/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/6/C1561    most recent
00562.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (20)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pedersen, S. F.
Right arrow Articles by Cala, P. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pedersen, S. F.
Right arrow Articles by Cala, P. M.
Vol. 284, Issue 6, C1561-C1576, June 2003

Molecular cloning of NHE1 from winter flounder RBCs: activation by osmotic shrinkage, cAMP, and calyculin A

Stine F. Pedersen1, Scott A. King1, Robert R. Rigor1, Zhenpeng Zhuang1, Jaimie M. Warren2, and Peter M. Cala1

1 Department of Human Physiology, School of Medicine, University of California-Davis, and 2 United States Department of Agriculture-Agricultural Research Service Western Human Nutrition Research Center, Davis, California 95616

In this report, we describe the cloning, cellular localization, and functional characteristics of Na+/H+ exchanger 1 (NHE1) from red blood cells of the winter flounder Pseudopleuronectes americanus (paNHE1). The paNHE1 protein localizes primarily to the marginal band and exhibits a 74% similarity to the trout beta -NHE, and 65% to the human NHE1 (hNHE1). Functionally, paNHE1 shares characteristics of both beta -NHE and hNHE1 in that it is activated both by manipulations that increase cAMP and by cell shrinkage, respectively. In accordance, the paNHE1 protein exhibits both protein kinase A consensus sites as in beta -NHE and a region of high homology to that required for shrinkage-dependent activation of hNHE1. After shrinkage-dependent activation of paNHE1 and resulting activation of a Cl-/HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> exchanger, their parallel operation results in net uptake of NaCl and osmotically obliged water. Activation of paNHE1 by cAMP is at least additive to that elicited by osmotic shrinkage, suggesting that these stimuli regulate paNHE1 by distinct mechanisms. Finally, exposure to the serine/threonine phosphatase inhibitor calyculin A potently activates paNHE1, and this activation is also additive to that induced by shrinkage or cAMP.

sodium-proton antiport; red blood cells; beta -Na+/H+ exchanger; protein kinase A; protein kinase C; protein phosphatases


This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
E. K. Hoffmann, I. H. Lambert, and S. F. Pedersen
Physiology of Cell Volume Regulation in Vertebrates
Physiol Rev, January 1, 2009; 89(1): 193 - 277.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. Mandal, N. A. Delamere, and M. Shahidullah
Ouabain-induced stimulation of sodium-hydrogen exchange in rat optic nerve astrocytes
Am J Physiol Cell Physiol, July 1, 2008; 295(1): C100 - C110.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. Rivera, L. De Franceschi, L. L. Peters, P. Gascard, N. Mohandas, and C. Brugnara
Effect of complete protein 4.1R deficiency on ion transport properties of murine erythrocytes
Am J Physiol Cell Physiol, November 1, 2006; 291(5): C880 - C886.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
K. H. Ahmed, B. Pelster, and G. Krumschnabel
Signalling pathways involved in hypertonicity- and acidification-induced activation of Na+/H+ exchange in trout hepatocytes
J. Exp. Biol., August 15, 2006; 209(16): 3101 - 3113.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
S. F. Pedersen, M. E. O'Donnell, S. E. Anderson, and P. M. Cala
Physiology and pathophysiology of Na+/H+ exchange and Na+-K+-2Cl- cotransport in the heart, brain, and blood
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2006; 291(1): R1 - R25.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
R. M. Pelis, S. L. Edwards, S. C. Kunigelis, J. B. Claiborne, and J. L. Renfro
Stimulation of renal sulfate secretion by metabolic acidosis requires Na+/H+ exchange induction and carbonic anhydrase
Am J Physiol Renal Physiol, July 1, 2005; 289(1): F208 - F216.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. L. Brett, M. Donowitz, and R. Rao
Evolutionary origins of eukaryotic sodium/proton exchangers
Am J Physiol Cell Physiol, February 1, 2005; 288(2): C223 - C239.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2003 by the American Physiological Society.