Am J Physiol Cell Physiol AJP: Advances in Physiology Education
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 284: C1460-C1467, 2003. First published February 26, 2003; doi:10.1152/ajpcell.00603.2002
0363-6143/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/6/C1460    most recent
00603.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (15)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Parkerson, K. A.
Right arrow Articles by Sontheimer, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Parkerson, K. A.
Right arrow Articles by Sontheimer, H.
Vol. 284, Issue 6, C1460-C1467, June 2003

Contribution of chloride channels to volume regulation of cortical astrocytes

Kimberly A. Parkerson and Harald Sontheimer

Department of Neurobiology, Civitan International Research Center, University of Alabama at Birmingham, Birmingham, Alabama 35294

The objective of this study was to determine the relative contribution of Cl- channels to volume regulation of cultured rat cortical astrocytes after hypotonic cell swelling. Using a Coulter counter, we showed that cortical astrocytes regulate their cell volume by ~60% within 45 min after hypotonic challenge. This volume regulation was supported when Cl- was replaced with Br-, NO<UP><SUB>3</SUB><SUP>−</SUP></UP>, methanesulfonate-, or acetate- but was inhibited when Cl- was replaced with isethionate- or gluconate-. Additionally, substitution of Cl- with I- completely blocked volume regulation. Volume regulation was unaffected by furosemide or bumetanide, blockers of KCl transport, but was inhibited by Cl- channel blockers, including 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB), 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), and niflumic acid. Surprisingly, the combination of Cd2+ with NPPB, DIDS, or niflumic acid inhibited regulation to a greater extent than any of these drugs alone. Volume regulation did not differ among astrocytes cultured from different brain regions, as cerebellar and hippocampal astrocytes exhibited behavior identical to that of cortical astrocytes. These data suggest that Cl- flux through ion channels rather than transporters is essential for volume regulation of cultured astrocytes in response to hypotonic challenge.

glia; ion channels; Coulter counter


This article has been cited by other articles:


Home page
 Exp. Biol. Med.Home page
H. Sontheimer
An Unexpected Role for Ion Channels in Brain Tumor Metastasis
Experimental Biology and Medicine, July 1, 2008; 233(7): 779 - 791.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
L. Chi-Wei, S.-L. Chang, and C.-F. Weng
Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Regulates the Expression of PACAP in Cultured Tilapia Astrocytes
Experimental Biology and Medicine, February 1, 2007; 232(2): 262 - 276.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
N. J. Ernest, A. K. Weaver, L. B. Van Duyn, and H. W. Sontheimer
Relative contribution of chloride channels and transporters to regulatory volume decrease in human glioma cells
Am J Physiol Cell Physiol, June 1, 2005; 288(6): C1451 - C1460.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2003 by the American Physiological Society.