Am J Physiol Cell Physiol AJP: Cell Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 284: C1420-C1428, 2003. First published February 26, 2003; doi:10.1152/ajpcell.00556.2002
0363-6143/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/6/C1420    most recent
00556.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (31)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Abaffy, T.
Right arrow Articles by Chaudhari, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Abaffy, T.
Right arrow Articles by Chaudhari, N.
Vol. 284, Issue 6, C1420-C1428, June 2003

Adenylyl cyclase expression and modulation of cAMP in rat taste cells

Tatjana Abaffy, Kristina R. Trubey, and Nirupa Chaudhari

Department of Physiology and Biophysics, University of Miami School of Medicine, Miami, Florida 33101

cAMP is a second messenger implicated in sensory transduction for taste. The identity of adenylyl cyclase (AC) in taste cells has not been explored. We have employed RT-PCR to identify the AC isoforms present in taste cells and found that AC 4, 6, and 8 are expressed as mRNAs in taste tissue. These proteins are also expressed in a subset of taste cells as revealed by immunohistochemistry. Alterations of cAMP concentrations are associated with transduction of taste stimuli of several classes. The involvement of particular ACs in this modulation has not been investigated. We demonstrate that glutamate, which is a potent stimulus eliciting a taste quality termed umami, causes a decrease in cAMP in forskolin-treated taste cells. The potentiation of this response by inosine monophosphate, the lack of response to D-glutamate, and the lack of response to umami stimuli in nonsensory lingual epithelium all suggest that the cAMP modulation represents umami taste transduction. Because cAMP downregulation via ACs can be mediated through Galpha i proteins, we examined the colocalization of the detected ACs with Galpha i proteins and found that 66% of AC8 immunopositive taste cells are also positive for gustducin, a taste-specific Galpha i protein. Whether AC8 is directly involved in signal transduction of umami taste remains to be established.

immunohistochemistry; glutamate; umami; taste transduction; gustducin


This article has been cited by other articles:


Home page
 Am. J. Clin. Nutr.Home page
N. Chaudhari, E. Pereira, and S. D Roper
Taste receptors for umami: the case for multiple receptors
Am. J. Clinical Nutrition, September 1, 2009; 90(3): 738S - 742S.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
A. San Gabriel, T. Maekawa, H. Uneyama, and K. Torii
Metabotropic glutamate receptor type 1 in taste tissue
Am. J. Clinical Nutrition, September 1, 2009; 90(3): 743S - 746S.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
M. Raliou, A. Wiencis, A.-M. Pillias, A. Planchais, C. Eloit, Y. Boucher, D. Trotier, J.-P. Montmayeur, and A. Faurion
Nonsynonymous single nucleotide polymorphisms in human tas1r1, tas1r3, and mGluR1 and individual taste sensitivity to glutamate
Am. J. Clinical Nutrition, September 1, 2009; 90(3): 789S - 799S.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
C. D. Roberts, G. Dvoryanchikov, S. D. Roper, and N. Chaudhari
Interaction between the second messengers cAMP and Ca2+ in mouse presynaptic taste cells
J. Physiol., April 15, 2009; 587(8): 1657 - 1668.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Interv.Home page
R. K. Palmer
The Pharmacology and Signaling of Bitter, Sweet, and Umami Taste Sensing
Mol. Interv., April 1, 2007; 7(2): 87 - 98.
[Abstract] [Full Text] [PDF]

Home page
 Chem SensesHome page
C. Bezencon, J. le Coutre, and S. Damak
Taste-Signaling Proteins Are Coexpressed in Solitary Intestinal Epithelial Cells
Chem Senses, January 1, 2007; 32(1): 41 - 49.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
K. R. Trubey, S. Culpepper, Y. Maruyama, S. C. Kinnamon, and N. Chaudhari
Tastants evoke cAMP signal in taste buds that is independent of calcium signaling
Am J Physiol Cell Physiol, August 1, 2006; 291(2): C237 - C244.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
Y. Maruyama, E. Pereira, R. F. Margolskee, N. Chaudhari, and S. D. Roper
Umami responses in mouse taste cells indicate more than one receptor.
J. Neurosci., February 22, 2006; 26(8): 2227 - 2234.
[Abstract] [Full Text] [PDF]

Home page
 Chem SensesHome page
N. Chaudhari, Y. Maruyama, S. Roper, and K. Trubey
Multiple Pathways for Signaling Glutamate Taste in Rodents
Chem Senses, January 1, 2005; 30(suppl_1): i29 - i30.
[Full Text] [PDF]

Home page
 Chem SensesHome page
B. R. Heyer, C. C. Taylor-Burds, J. D. Mitzelfelt, and E. R. Delay
Monosodium Glutamate and Sweet Taste: Discrimination between the Tastes of Sweet Stimuli and Glutamate in Rats
Chem Senses, October 1, 2004; 29(8): 721 - 729.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
W. He, K. Yasumatsu, V. Varadarajan, A. Yamada, J. Lem, Y. Ninomiya, R. F. Margolskee, and S. Damak
Umami Taste Responses Are Mediated by {alpha}-Transducin and {alpha}-Gustducin
J. Neurosci., September 1, 2004; 24(35): 7674 - 7680.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2003 by the American Physiological Society.