Am J Physiol Cell Physiol Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 284: C1355-C1361, 2003. First published January 15, 2003; doi:10.1152/ajpcell.00224.2002
0363-6143/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/6/C1355    most recent
00224.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (32)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pithon-Curi, T. C.
Right arrow Articles by Curi, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pithon-Curi, T. C.
Right arrow Articles by Curi, R.
Vol. 284, Issue 6, C1355-C1361, June 2003

Glutamine delays spontaneous apoptosis in neutrophils

Tania C. Pithon-Curi1, Robert Ivan Schumacher2, Jofre J. S. Freitas3, Cláudia Lagranha3, Philip Newsholme4, Adrianne C. Palanch1, Sonia Q. Doi5, and Rui Curi3

1 Methodist University of Piracicaba, FACEF, and Camilo Castelo Branco University, São Paulo; 2 Department of Biochemistry and 3 Department of Physiology and Biophysics, University of São Paulo, São Paulo, SP, Brazil; 4 Department of Biochemistry, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland; and 5 Uniformed Services University, Bethesda, Maryland 20814

Nuclear, mitochondrial, and plasma membrane events associated with apoptosis were investigated in rat neutrophils cultivated for 3, 24, and 48 h in the absence or presence of glutamine (0.5, 1.0, and 2.0 mM). Condensation of chromatin was reduced after 24 or 48 h of culture in the presence of glutamine compared with its absence as assessed by Hoechst 33342 staining. The level of Escherichia coli phagocytosis in the presence of glutamine was markedly increased compared with the level achieved by cells cultured in the absence of glutamine. Annexin V binding to externalized phosphatidylserine was reduced in the presence of glutamine. Sensitive fluorochrome rhodamine 123, as determined by fluorescence-activated cell sorting and confocal microscopy, was used to monitor loss of the mitochondrial transmembrane potential. In the absence of glutamine, neutrophils exhibited a marked reduction in the uptake of rhodamine 123. In the presence of 1.0 or 2.0 mM glutamine, the uptake of rhodamine was 20 or 38% higher, respectively. Similar effect was found in human neutrophils by measuring DNA fragmentation and mitochondrial transmembrane potential. Therefore, glutamine protects from events associated with triggering and executing apoptosis in both rat and human neutrophils.

mitochondria; neutrophil


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Y.-C. Hou, M.-H. Pai, W.-C. Chiu, Y.-M. Hu, and S.-L. Yeh
Effects of dietary glutamine supplementation on lung injury induced by lipopolysaccharide administration
Am J Physiol Lung Cell Mol Physiol, March 1, 2009; 296(3): L288 - L295.
[Abstract] [Full Text] [PDF]

Home page
 JPEN J Parenter Enteral NutrHome page
M. F. Cury-Boaventura, R. Gorjao, T. M. de Lima, T. M. Piva, C. M. Peres, F. G. Soriano, and R. Curi
Toxicity of a Soybean Oil Emulsion on Human Lymphocytes and Neutrophils
JPEN J Parenter Enteral Nutr, March 1, 2006; 30(2): 115 - 123.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
B. A. Carneiro, J. Fujii, G. A. C. Brito, C. Alcantara, R. B. Oria, A. A. M. Lima, T. Obrig, and R. L. Guerrant
Caspase and Bid Involvement in Clostridium difficile Toxin A-Induced Apoptosis and Modulation of Toxin A Effects by Glutamine and Alanyl-Glutamine In Vivo and In Vitro
Infect. Immun., January 1, 2006; 74(1): 81 - 87.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2003 by the American Physiological Society.