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Am J Physiol Cell Physiol 284: C1133-C1139, 2003. First published December 27, 2002; doi:10.1152/ajpcell.00293.2002
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Vol. 284, Issue 5, C1133-C1139, May 2003

Interferon-gamma induces C/EBPbeta expression and activity through MEK/ERK and p38 in T84 colon epithelial cells

Pertteli Salmenperä1, Sammy Hämäläinen1, Mika Hukkanen2, and Esko Kankuri1

Departments of 1 Pharmacology and 2 Anatomy, Institute of Biomedicine, FIN-00014 University of Helsinki, Finland

We investigated the role of IFN-gamma and MAPKs on the expression and activity of the transcription factor CCAAT/enhancer-binding protein-beta (C/EBPbeta ) in the T84 colon epithelial cell line. IFN-gamma induced the expression and activity of C/EBPbeta and subsequently increased the secretion of IL-6 from these cells. Treatment with the p38 inhibitor SB-203580, the MEK1 and MEK2 inhibitor U-0126, or the translational inhibitor cycloheximide inhibited the induction of C/EBPbeta and IL-6 by IFN-gamma , whereas the MEK1 inhibitor PD-98059 or the tyrosine kinase inhibitor genistein had no effect. These results suggest a role for MEK2 and p38 in IFN-gamma -mediated signal transduction and induction of C/EBPbeta expression and activity associated with interleukin-6 (IL-6) secretion in colon epithelial cells.

CCAAT-enhancer binding protein-beta ; colon epithelial cells, interferon-gamma ; interleukin-6; mitogen-activated protein kinases




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