Am J Physiol Cell Physiol AJP: Heart and Circulatory Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 284: C718-C728, 2003. First published November 20, 2002; doi:10.1152/ajpcell.00359.2002
0363-6143/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/3/C718    most recent
00359.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (28)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Blumenthal, E. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Blumenthal, E. M.
Vol. 284, Issue 3, C718-C728, March 2003

Regulation of chloride permeability by endogenously produced tyramine in the Drosophila Malpighian tubule

Edward M. Blumenthal

Department of Biology and Center for Biological Timing, University of Virginia, Charlottesville, Virginia 22904-4328

The Malpighian (renal) tubule of Drosophila melanogaster is a useful model for studying epithelial transport. The purpose of this study was to identify factors responsible for modulating transepithelial chloride conductance in isolated tubules. I have found that tyrosine and several of its metabolites cause an increase in chloride conductance. The most potent of these agonists is tyramine, which is active at low nanomolar concentrations; the pharmacology of this response matches that of the previously published cloned insect tyramine receptor. In addition, the tubule appears capable of synthesizing tyramine from applied tyrosine, as shown by direct measurement of tyrosine decarboxylase activity. Immunohistochemical staining of tubules with an antibody against tyramine indicates that the principal cells are the sites of tyramine production, whereas previous characterization of the regulation of chloride conductance suggests that tyramine acts on the stellate cells. This is the first demonstration of a physiological role for an insect tyramine receptor.

tyrosine decarboxylase; yohimbine; tyramine receptor; biogenic amines


This article has been cited by other articles:


Home page
 J. Exp. Biol.Home page
E. M. Blumenthal
Isoform- and cell-specific function of tyrosine decarboxylase in the Drosophila Malpighian tubule
J. Exp. Biol., December 1, 2009; 212(23): 3802 - 3809.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
J. A. T. Dow
Insights into the Malpighian tubule from functional genomics
J. Exp. Biol., February 1, 2009; 212(3): 435 - 445.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
B. Brembs, F. Christiansen, H. J. Pfluger, and C. Duch
Flight Initiation and Maintenance Deficits in Flies with Genetically Altered Biogenic Amine Levels
J. Neurosci., October 10, 2007; 27(41): 11122 - 11131.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
E. M. Blumenthal
Modulation of tyramine signaling by osmolality in an insect secretory epithelium
Am J Physiol Cell Physiol, November 1, 2005; 289(5): C1261 - C1267.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. H. Cole, G. E. Carney, C. A. McClung, S. S. Willard, B. J. Taylor, and J. Hirsh
Two Functional but Noncomplementing Drosophila Tyrosine Decarboxylase Genes: DISTINCT ROLES FOR NEURAL TYRAMINE AND OCTOPAMINE IN FEMALE FERTILITY
J. Biol. Chem., April 15, 2005; 280(15): 14948 - 14955.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online