Am J Physiol Cell Physiol Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 284: C705-C717, 2003. First published November 13, 2002; doi:10.1152/ajpcell.00331.2002
0363-6143/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/3/C705    most recent
00331.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (9)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Green, C. E.
Right arrow Articles by Simon, S. I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Green, C. E.
Right arrow Articles by Simon, S. I.
Vol. 284, Issue 3, C705-C717, March 2003

Topographic requirements and dynamics of signaling via L-selectin on neutrophils

Chad E. Green, David N. Pearson, Nadine B. Christensen, and Scott I. Simon

Department of Biomedical Engineering, University of California, Davis, California 95616

Cross-linking of L-selectin on leukocytes signals phosphorylation of mitogen-activated protein kinases (MAPKs) leading to activation of CD18 function and enhanced transmigration on inflamed endothelium. We examined how alterations in the topography of L-selectin correlate with the dynamics of CD18 activation and phosphorylation of MAPK. Simultaneous ligation of humanized antibodies DREG55 and DREG200 provided a strategy for regulating the extent of cross-linking. Triggering of CD11b/CD18 upregulation and adhesion required clustering of L-selectin to microvillus-sized patches of ~0.2 µm2. Immunofluorescence revealed that L-selectin was colocalized with high-affinity CD18. Anti-L-selectin-coated protein A microspheres indicated that a single site of contact to a 5.5-µm bead, or multiple contacts to 0.94- or 0.3-µm beads, elicited maximum neutrophil activation. Adhesion signaled via L-selectin coincided with the kinetics of MAPK phosphorylation and was inhibited by blocking p38 or p42/44 activity. These data demonstrate the capacity of L-selectin to transduce signals effecting rapid (~1 s) neutrophil adhesion that is regulated by the size and frequency of receptor clustering.

protein kinases; adhesion molecules; antibodies; cellular activation


This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
U. Schaff, P. E. Mattila, S. I. Simon, and B. Walcheck
Neutrophil adhesion to E-selectin under shear promotes the redistribution and co-clustering of ADAM17 and its proteolytic substrate L-selectin
J. Leukoc. Biol., January 1, 2008; 83(1): 99 - 105.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Lee, J. B. Schultz, P. A. Knauf, and M. R. King
Mechanical Shedding of L-selectin from the Neutrophil Surface during Rolling on Sialyl Lewis x under Flow
J. Biol. Chem., February 16, 2007; 282(7): 4812 - 4820.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
C. Chen, X. Ba, T. Xu, L. Cui, S. Hao, and X. Zeng
c-Abl Is Involved in the F-Actin Assembly Triggered by L-Selectin Crosslinking
J. Biochem., August 1, 2006; 140(2): 229 - 235.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
P. E. Mattila, C. E. Green, U. Schaff, S. I. Simon, and B. Walcheck
Cytoskeletal interactions regulate inducible L-selectin clustering
Am J Physiol Cell Physiol, August 1, 2005; 289(2): C323 - C332.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. E. Green, D. N. Pearson, R. T. Camphausen, D. E. Staunton, and S. I. Simon
Shear-Dependent Capping of L-Selectin and P-Selectin Glycoprotein Ligand 1 by E-Selectin Signals Activation of High-Avidity {beta}2-Integrin on Neutrophils
J. Immunol., June 15, 2004; 172(12): 7780 - 7790.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online