Am J Physiol Cell Physiol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 284: C681-C695, 2003. First published October 23, 2002; doi:10.1152/ajpcell.00370.2002
0363-6143/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/3/C681    most recent
00370.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (18)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Robinson, P. A.
Right arrow Articles by Mitchell, C. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Robinson, P. A.
Right arrow Articles by Mitchell, C. A.
Vol. 284, Issue 3, C681-C695, March 2003

Skeletal muscle LIM protein 1 regulates integrin-mediated myoblast adhesion, spreading, and migration

Paul A. Robinson*, Susan Brown*, Meagan J. McGrath, Imogen D. Coghill, Rajendra Gurung, and Christina A. Mitchell

Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia 3168

The skeletal muscle LIM protein 1 (SLIM1) is highly expressed in skeletal and cardiac muscle, and its expression is downregulated significantly in dilated human cardiomyopathy. However, the function of SLIM1 is unknown. In this study, we investigated the intracellular localization of SLIM1. Endogenous and recombinant SLIM1 localized to the nucleus, stress fibers, and focal adhesions in skeletal myoblasts plated on fibronectin, collagen, or laminin. However, after inhibition of integrin signaling either by plating on poly-L-lysine or by soluble RGD peptide, SLIM1 localized diffusely in the cytosol, with decreased nuclear expression. Disruption of the actin cytoskeleton by cytochalasin D did not inhibit nuclear localization of SLIM1 in integrin-activated cells. Green fluorescent protein-tagged SLIM1 shuttled in the nucleus of untransfected NIH 3T3 cells, in a heterokaryon fusion assay. Overexpression of SLIM1 in Sol8 myoblasts inhibited cell adhesion and promoted cell spreading and migration. These studies show SLIM1 localizes in an integrin-dependent manner to the nucleus and focal adhesions where it functions downstream of integrin activation to promote cell spreading and migration.

myoblast; integrins; LIM proteins; migration

* P. A. Robinson and S. Brown contributed equally to this work.




This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
C.-n. Chen, D. A. Ferrington, and L. V. Thompson
Carbonic anhydrase III and four-and-a-half LIM protein 1 are preferentially oxidized with muscle unloading
J Appl Physiol, November 1, 2008; 105(5): 1554 - 1561.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
Z. Yang, C. F. Browning, H. Hallaq, L. Yermalitskaya, J. Esker, M. R. Hall, A. J. Link, A.-J. L. Ham, M. J. McGrath, C. A. Mitchell, et al.
Four and a half LIM protein 1: a partner for KCNA5 in human atrium
Cardiovasc Res, June 1, 2008; 78(3): 449 - 457.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. L. Cottle, M. J. McGrath, B. S. Cowling, I. D. Coghill, S. Brown, and C. A. Mitchell
FHL3 binds MyoD and negatively regulates myotube formation
J. Cell Sci., April 15, 2007; 120(8): 1423 - 1435.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
A. Raffaello, P. Laveder, C. Romualdi, C. Bean, L. Toniolo, E. Germinario, A. Megighian, D. Danieli-Betto, C. Reggiani, and G. Lanfranchi
Denervation in murine fast-twitch muscle: short-term physiological changes and temporal expression profiling
Physiol Genomics, March 13, 2006; 25(1): 60 - 74.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. M. Samarel
Costameres, focal adhesions, and cardiomyocyte mechanotransduction
Am J Physiol Heart Circ Physiol, December 1, 2005; 289(6): H2291 - H2301.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. C. Mistry, A. Kato, Y. H. Tran, S. Honda, T. Tsukada, Y. Takei, and S. Hirose
FHL5, a novel actin-binding protein, is highly expressed in eel gill pillar cells and responds to wall tension
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2004; 287(5): R1141 - R1154.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. J. McGrath, C. A. Mitchell, I. D. Coghill, P. A. Robinson, and S. Brown
Skeletal muscle LIM protein 1 (SLIM1/FHL1) induces {alpha}5{beta}1-integrin-dependent myocyte elongation
Am J Physiol Cell Physiol, December 1, 2003; 285(6): C1513 - C1526.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online