| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Center for Cardiovascular Sciences, Albany Medical College, Albany 12208; and 2 Department of Biomedical Sciences, Cornell University, Ithaca, New York 14853
We examined the effects
of metabolic inhibition on intracellular Ca2+ release in
single pulmonary arterial smooth muscle cells (PASMCs). Severe
metabolic inhibition with cyanide (CN, 10 mM) increased intracellular
calcium concentration ([Ca2+]i) and activated
Ca2+-activated Cl
currents
[ICl(Ca)] in PASMCs, responses that were greatly
inhibited by BAPTA-AM or caffeine. Mild metabolic inhibition with CN (1 mM) increased spontaneous transient inward currents and
Ca2+ sparks in PASMCs. In Xenopus oocytes, CN
also induced Ca2+ release and activated
ICl(Ca), and these responses were inhibited by thapsigargin
and cyclopiazonic acid to deplete sarcoplasmic reticulum (SR)
Ca2+, whereas neither heparin nor anti-inositol
1,4,5-trisphosphate receptor (IP3R) antibodies affected CN
responses. In both PASMCs and oocytes, CN-evoked Ca2+
release was inhibited by carbonyl cyanide
m-chlorophenylhydrazone (CCCP) and oligomycin or CCCP and
thapsigargin. Whereas hypoxic stimuli resulted in Ca2+
release in pulmonary but not mesenteric artery myocytes, CN induced release in both cell types. We conclude that metabolic inhibition with
CN increases [Ca2+]i in both pulmonary and
systemic artery myocytes by stimulating Ca2+ release from
the SR and mitochondria.
sarcoplasmic reticulum; mitochondria
This article has been cited by other articles:
|
|
 |
|
  J. Wang, L. Weigand, J. Foxson, L. A. Shimoda, and J. T. Sylvester Ca2+ signaling in hypoxic pulmonary vasoconstriction: effects of myosin light chain and Rho kinase antagonists Am J Physiol Lung Cell Mol Physiol, September 1, 2007; 293(3): L674 - L685. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Caplanusi, A. J. Fuller, R. A. Gonzalez-Villalobos, T. G. Hammond, and L. G. Navar Metabolic inhibition-induced transient Ca2+ increase depends on mitochondria in a human proximal renal cell line Am J Physiol Renal Physiol, August 1, 2007; 293(2): F533 - F540. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. T. Ward Point:Counterpoint: Hypoxic pulmonary vasoconstriction is/is not mediated by increased production of reactive oxygen species J Appl Physiol, September 1, 2006; 101(3): 993 - 995. [Full Text] [PDF]
|
|
|
|
 |
|
 G. Ji, M. Feldman, R. Doran, W. Zipfel, and M. I. Kotlikoff Ca2+-Induced Ca2+ Release through Localized Ca2+ Uncaging in Smooth Muscle J. Gen. Physiol., February 27, 2006; 127(3): 225 - 235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-R. Yang, M.-J. Lin, K.-P. Yip, L. H. Jeyakumar, S. Fleischer, G. P. H. Leung, and J. S. K. Sham Multiple ryanodine receptor subtypes and heterogeneous ryanodine receptor-gated Ca2+ stores in pulmonary arterial smooth muscle cells Am J Physiol Lung Cell Mol Physiol, August 1, 2005; 289(2): L338 - L348. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. O. Ibe, A. M. Portugal, S. Chaturvedi, and J. U. Raj Oxygen-dependent PAF receptor binding and intracellular signaling in ovine fetal pulmonary vascular smooth muscle Am J Physiol Lung Cell Mol Physiol, May 1, 2005; 288(5): L879 - L886. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-M. Zheng, Q.-S. Wang, R. Rathore, W.-H. Zhang, J. E. Mazurkiewicz, V. Sorrentino, H. A. Singer, M. I. Kotlikoff, and Y.-X. Wang Type-3 Ryanodine Receptors Mediate Hypoxia-, but Not Neurotransmitter-induced Calcium Release and Contraction in Pulmonary Artery Smooth Muscle Cells J. Gen. Physiol., March 28, 2005; 125(4): 427 - 440. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. B. Waypa and P. T. Schumacker Hypoxic pulmonary vasoconstriction: redox events in oxygen sensing J Appl Physiol, January 1, 2005; 98(1): 404 - 414. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wang, L. A. Shimoda, and J. T. Sylvester Capacitative calcium entry and TRPC channel proteins are expressed in rat distal pulmonary arterial smooth muscle Am J Physiol Lung Cell Mol Physiol, April 1, 2004; 286(4): L848 - L858. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Fantozzi, S. Zhang, O. Platoshyn, C. V. Remillard, R. T. Cowling, and J. X.-J. Yuan Hypoxia increases AP-1 binding activity by enhancing capacitative Ca2+ entry in human pulmonary artery endothelial cells Am J Physiol Lung Cell Mol Physiol, December 1, 2003; 285(6): L1233 - L1245. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Akimoto and M. R. Hammerman Microvessel formation from mouse aorta is stimulated in vitro by secreted VEGF and extracts from metanephroi Am J Physiol Cell Physiol, June 1, 2003; 284(6): C1625 - C1632. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
