Am J Physiol Cell Physiol AJP: Heart and Circulatory Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 284: C77-C84, 2003. First published September 11, 2002; doi:10.1152/ajpcell.00132.2002
0363-6143/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/1/C77    most recent
00132.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (32)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, J.
Right arrow Articles by Okada, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, J.
Right arrow Articles by Okada, Y.
Vol. 284, Issue 1, C77-C84, January 2003

IK channels are involved in the regulatory volume decrease in human epithelial cells

Jun Wang, Shigeru Morishima, and Yasunobu Okada

Department of Cell Physiology, National Institute for Physiological Sciences; and Core Research for Evolutional Science and Technology of Japan Science and Technology Corporation, Okazaki 444-8585, Japan

Parallel activation of Ca2+-dependent K+ channels and volume-sensitive Cl- channels is known to be responsible for KCl efflux during regulatory volume decrease (RVD) in human epithelial Intestine 407 cells. The present study was performed to identify the K+ channel type. RT-PCR demonstrated mRNA expression of Ca2+-activated, intermediate conductance K+ (IK), but not small conductance K+ (SK1) or large conductance K+ (BK) channels in this cell line. Whole cell recordings showed that ionomycin or hypotonic stress activated inwardly rectifying K+ currents that were reversibly blocked by IK channel blockers [clotrimazole (CLT) and charybdotoxin] but not by SK and BK channel blockers (apamin and iberiotoxin). Inside-out recordings revealed the existence of CLT-sensitive single K+-channel activity, which exhibited an intermediate unitary conductance (30 pS at -100 mV). The channel was activated by cytosolic Ca2+ in inside-out patches and by a hypotonic challenge in cell-attached patches. The RVD was suppressed by CLT, but not by apamin or iberiotoxin. Thus we conclude that the IK channel is involved in the RVD process in these human epithelial cells.

Ca2+-activated K+ channel; patch clamp; clotrimazole; osmotic swelling


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
E. L. Lee, Y. Hasegawa, T. Shimizu, and Y. Okada
IK1 channel activity contributes to cisplatin sensitivity of human epidermoid cancer cells
Am J Physiol Cell Physiol, June 1, 2008; 294(6): C1398 - C1406.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
P. K. Lauf, S. Misri, A. A. Chimote, and N. C. Adragna
Apparent intermediate K conductance channel hyposmotic activation in human lens epithelial cells
Am J Physiol Cell Physiol, March 1, 2008; 294(3): C820 - C832.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. T. Halm, T. Liao, and D. R. Halm
Distinct K+ conductive pathways are required for Cl- and K+ secretion across distal colonic epithelium
Am J Physiol Cell Physiol, October 1, 2006; 291(4): C636 - C648.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. W. Musch and L. Goldstein
Tyrosine kinase inhibition affects skate anion exchanger isoform I alterations after volume expansion
Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2005; 288(4): R885 - R890.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
Y. Ito, M. Son, S. Sato, T. Ishikawa, M. Kondo, S. Nakayama, K. Shimokata, and H. Kume
ATP Release Triggered by Activation of the Ca2+-Activated K+ Channel in Human Airway Calu-3 Cells
Am. J. Respir. Cell Mol. Biol., March 1, 2004; 30(3): 388 - 395.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. Hayashi, C. Kunii, T. Takahata, and T. Ishikawa
ATP-dependent regulation of SK4/IK1-like currents in rat submandibular acinar cells: possible role of cAMP-dependent protein kinase
Am J Physiol Cell Physiol, March 1, 2004; 286(3): C635 - C646.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. V. Remillard and J. X.-J. Yuan
Activation of K+ channels: an essential pathway in programmed cell death
Am J Physiol Lung Cell Mol Physiol, January 1, 2004; 286(1): L49 - L67.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online