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Am J Physiol Cell Physiol 284: C233-C241, 2003. First published September 25, 2002; doi:10.1152/ajpcell.00087.2002
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Vol. 284, Issue 1, C233-C241, January 2003

Cellular distributions of creatine kinase in branchia of euryhaline tilapia (Oreochromis mossambicus)

Li-Yih Lin1,2, Chia-Chang Chiang2, Hong-Yi Gong1,2, Ching-Yi Cheng2,3, Pung-Pung Hwang1,2,*, and Ching-Feng Weng3,*

1 Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan 100; 2 Institute of Zoology, Academia Sinica, Taipei, Taiwan 115; and 3 Institute of Biotechnology, National Dong Hwa University, Hualien, Taiwan 974, Republic of China

Although euryhaline teleosts can adapt to environmental fluctuation of salinity, their energy source for responding to changes in salinity and osmolarity remains unclear. This study examines the cellular localization of creatine kinase (CK) expression in branchia of tilapia (Oreochromis mossambicus). Western blot analysis of muscle-type CK (MM form) revealed a high association with salinity changes, but BB and MB forms of CK in the gills of fish adapted to seawater did not change. With the use of immunocytochemistry, three CK isoforms (MM, MB, and BB) were localized in mitochondria-rich (MR) cells and other epithelial cells of tilapia gills. In addition, staining intensity of MM-form CK in MR cells increased after seawater transfer, whereas BB and MB forms did not significantly change. To our knowledge, this work presents the first evidence of CK expression in MR cells of tilapia gills, highlighting the potential role of CK in providing energy for ion transport.

creatine kinase isoform; mitochondria-rich cells; gill

* P.-P. Hwang and C.-F. Weng contributed equally to this work. Address for reprint requests and other correspondence: C.-F. Weng, Institute of Biotechnology, National Dong Hwa Univ., Hualien, Taiwan 974, ROC (E-mail:cfweng{at}mail.ndhu.edu.tw).




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