Am J Physiol Cell Physiol Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 283: C1795-C1800, 2002; doi:10.1152/ajpcell.00186.2002
0363-6143/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (19)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Buyse, M.
Right arrow Articles by Bado, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Buyse, M.
Right arrow Articles by Bado, A.
Vol. 283, Issue 6, C1795-C1800, December 2002

REPORT
PepT1-mediated fMLP transport induces intestinal inflammation in vivo

Marion Buyse1, Annick Tsocas2, Francine Walker2, Didier Merlin1, and Andre Bado2

1 Epithelial Pathology Unit, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322; and 2 Institut National de la Santé et de la Recherche Médicale Unité 410, Faculte de Medecine Xavier Bichat Paris, 75018 Paris, France

In the present study, the effect of H+/peptide transporter (PepT1)-mediated N-formylmethionyl-leucyl-phenylalanine (fMLP) transport on inflammation in vivo in the rat small intestine, which expresses high PepT1 levels, and in the rat colon, which does not express PepT1, were investigated using myeloperoxidase (MPO) activity and histological analysis. We found that 10 µM fMLP perfusion in the jejunum for 4 h significantly increased MPO activity and altered the architecture of jejunal villi. In contrast, 10 µM fMLP perfusion in the colon for 4 h did not induce any inflammation. In addition, we have shown that 50 mM Gly-Gly alone did not affect basal MPO activity but completely inhibited the MPO activity induced by 10 µM fMLP in the jejunum. Together, these experiments demonstrate that 1) the differential expression of PepT1 between the small intestine and the colon plays an important role in epithelial-neutrophil interactions and 2) the inhibition of fMLP uptake by jejunal epithelial cells (expressing PepT1) reduces the neutrophil ability to move across the epithelium, in agreement with our previously published in vitro study. This report constitutes the first in vivo study showing the implication of a membrane transporter (PepT1) in intestinal inflammation.

PepT1; N-formylmethionyl-leucyl-phenylalanine; myeloperoxidase; rat


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
R. M. Carlson, S. R. Vavricka, J. J. Eloranta, M. W. Musch, D. L. Arvans, K. A. Kles, M. M. Walsh-Reitz, G. A. Kullak-Ublick, and E. B. Chang
fMLP induces Hsp27 expression, attenuates NF-{kappa}B activation, and confers intestinal epithelial cell protection
Am J Physiol Gastrointest Liver Physiol, April 1, 2007; 292(4): G1070 - G1078.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
P. Plaisancie, R. Ducroc, M. E. Homsi, A. Tsocas, S. Guilmeau, S. Zoghbi, O. Thibaudeau, and A. Bado
Luminal leptin activates mucin-secreting goblet cells in the large bowel
Am J Physiol Gastrointest Liver Physiol, April 1, 2006; 290(4): G805 - G812.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. A. Adibi
Regulation of expression of the intestinal oligopeptide transporter (Pept-1) in health and disease
Am J Physiol Gastrointest Liver Physiol, November 1, 2003; 285(5): G779 - G788.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online