Am J Physiol Cell Physiol Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 283: C1637-C1645, 2002. First published August 14, 2002; doi:10.1152/ajpcell.00266.2002
0363-6143/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/6/C1637    most recent
00266.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (26)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Walker, R. L.
Right arrow Articles by Horowitz, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Walker, R. L.
Right arrow Articles by Horowitz, B.
Vol. 283, Issue 6, C1637-C1645, December 2002

TRPC4 currents have properties similar to the pacemaker current in interstitial cells of Cajal

Rebecca L. Walker*, Sang Don Koh*, Gerard P. Sergeant, Kenton M. Sanders, and Burton Horowitz

Department of Physiology and Cell Biology, University of Nevada, School of Medicine, Reno, Nevada 89557

Interstitial cells of Cajal (ICC) are the pacemaker cells responsible for the generation and propagation of electrical slow waves in phasic muscles of the gastrointestinal (GI) tract. The pacemaker current that initiates each slow wave derives from a calcium-inhibited, voltage-independent, nonselective cation channel. This channel in ICC displays properties similar to that reported for the transient receptor potential (TRP) family of nonselective cation channels, particularly those seen for TRPC3 and TRPC4. We have identified transcripts for TRPC4 in individually isolated ICC and have cloned the two alternatively spliced forms of TRPC4, TRPC4alpha and TRPC4beta , from GI muscles. TRPC4beta is missing an 84-amino acid segment from the carboxy terminus. Expression of either form using the whole cell patch-clamp technique led to calcium-inhibited, nonselective cation channels as determined by N-methyl-D-glucamine replacement experiments and BAPTA dialysis. Expression of TRPC4beta channels recorded at the whole cell level had characteristics similar to the nonselective cation current in ICC. The single-channel conductance of TRPC4beta was determined to be 17.5 pS. Application of calmidazolium to cells expressing TRPC4beta led to a significant increase in the inward current of these cells at both the whole cell and single-channel level, and currents were sensitive to block by 10 µM lanthanum, niflumic acid, and DIDS. Comparison of the properties reported for the nonselective cation current in ICC and those identified here for TRPC4beta led us to conclude that a TRPC4-like current encodes the plasmalemmal pacemaker current in murine small intestine.

cation channel; gastrointestinal; smooth muscle; calmodulin

* R. L. Walker and S. D. Koh contributed equally to this work.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
N. G. Jin, S. D. Koh, and K. M. Sanders
Caffeine inhibits nonselective cationic currents in interstitial cells of Cajal from the murine jejunum
Am J Physiol Cell Physiol, October 1, 2009; 297(4): C971 - C978.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
T. Collin, R. Franconville, B. E. Ehrlich, and I. Llano
Activation of Metabotropic Glutamate Receptors Induces Periodic Burst Firing and Concomitant Cytosolic Ca2+ Oscillations in Cerebellar Interneurons
J. Neurosci., July 22, 2009; 29(29): 9281 - 9291.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
H. Chen, T. Ordog, J. Chen, D. L. Young, M. R. Bardsley, D. Redelman, S. M. Ward, and K. M. Sanders
Differential gene expression in functional classes of interstitial cells of Cajal in murine small intestine
Physiol Genomics, November 14, 2007; 31(3): 492 - 509.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
Y.-K. Ju, Y. Chu, H. Chaulet, D. Lai, O. L. Gervasio, R. M. Graham, M. B. Cannell, and D. G. Allen
Store-Operated Ca2+ Influx and Expression of TRPC Genes in Mouse Sinoatrial Node
Circ. Res., June 8, 2007; 100(11): 1605 - 1614.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
Y. Zhu, J. Ye, and J. D. Huizinga
Clotrimazole-sensitive K+ currents regulate pacemaker activity in interstitial cells of Cajal
Am J Physiol Gastrointest Liver Physiol, June 1, 2007; 292(6): G1715 - G1725.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
S. Nakayama, K. Shimono, H.-N. Liu, H. Jiko, N. Katayama, T. Tomita, and K. Goto
Pacemaker phase shift in the absence of neural activity in guinea-pig stomach: a microelectrode array study
J. Physiol., November 1, 2006; 576(3): 727 - 738.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. T. Kim, B. J. Kim, J. H. Lee, S. C. Kwon, D. S. Yeon, D. K. Yang, I. So, and K. W. Kim
Involvement of calmodulin and myosin light chain kinase in activation of mTRPC5 expressed in HEK cells
Am J Physiol Cell Physiol, April 1, 2006; 290(4): C1031 - C1040.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
P. R. Strege, C. E. Bernard, Y. Ou, S. J. Gibbons, and G. Farrugia
Effect of mibefradil on sodium and calcium currents
Am J Physiol Gastrointest Liver Physiol, August 1, 2005; 289(2): G249 - G253.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
Y. Zhu, A. Mucci, and J. D. Huizinga
Inwardly rectifying chloride channel activity in intestinal pacemaker cells
Am J Physiol Gastrointest Liver Physiol, April 1, 2005; 288(4): G809 - G821.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. Yano, T. Ishikawa, H. Tsuda, K. Obara, and K. Nakayama
Ionic mechanism for contractile response to hyposmotic challenge in canine basilar arteries
Am J Physiol Cell Physiol, March 1, 2005; 288(3): C702 - C709.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
I. Fantozzi, S. Zhang, O. Platoshyn, C. V. Remillard, R. T. Cowling, and J. X.-J. Yuan
Hypoxia increases AP-1 binding activity by enhancing capacitative Ca2+ entry in human pulmonary artery endothelial cells
Am J Physiol Lung Cell Mol Physiol, December 1, 2003; 285(6): L1233 - L1245.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
S. G. Shlykov, M. Yang, J. L. Alcorn, and B. M. Sanborn
Capacitative Cation Entry in Human Myometrial Cells and Augmentation by hTrpC3 Overexpression
Biol Reprod, August 1, 2003; 69(2): 647 - 655.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online