| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Department of Bioengineering and Whitaker Institute of Biomedical Engineering, University of California at San Diego, La Jolla, California 92093
Blood flow can modulate vascular cell
functions. We studied interactions between integrins and Flk-1 in
transducing the mechanical shear stress due to flow. This application
of a step shear stress caused Flk-1 · Casitas B-lineage
lymphoma (Cbl) activation (Flk-1 · Cbl association, tyrosine
phosphorylation of the Cbl-bound Flk-1, and tyrosine phosphorylation of
Cbl) in bovine aortic endothelial cells (BAECs). The activation of
integrins by plating BAECs on vitronectin or fibronectin also induced
this Flk-1 · Cbl activation. The shear-induced
Flk-1 · Cbl activation was blocked by inhibitory antibodies for
v
3- or 1-integrin,
suggesting that it is mediated by integrins. Inhibition of Flk-1 by
SU1498 also abolished this shear-induced Flk-1 · Cbl
activation. In contrast to the requirement of integrins for
Flk-1 · Cbl activation, the Flk-1 blocker SU1498 had no
detectable effect on the shear-induced integrin activation, suggesting
that integrins and Flk-1 play sequential roles in the signal
transduction hierarchy induced by shear stress. Integrins are essential
for the mechanical activation of Flk-1 by shear stress but not for the
chemical activation of Flk-1 by VEGF.
mechanotransduction; vascular endothelial growth factor; Casitas B-lineage lymphoma
This article has been cited by other articles:
|
|
 |
|
  Y.-X. Qi, M.-J. Qu, D.-K. Long, B. Liu, Q.-P. Yao, S. Chien, and Z.-L. Jiang Rho-GDP dissociation inhibitor alpha downregulated by low shear stress promotes vascular smooth muscle cell migration and apoptosis: a proteomic analysis Cardiovasc Res, October 1, 2008; 80(1): 114 - 122. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Zheng, L. P. Christensen, and R. J. Tomanek Differential effects of cyclic and static stretch on coronary microvascular endothelial cell receptors and vasculogenic/angiogenic responses Am J Physiol Heart Circ Physiol, August 1, 2008; 295(2): H794 - H800. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chien Mechanotransduction and endothelial cell homeostasis: the wisdom of the cell Am J Physiol Heart Circ Physiol, March 1, 2007; 292(3): H1209 - H1224. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Weis, J. N. Lindquist, L. A. Barnes, K. M. Lutu-Fuga, J. Cui, M. R. Wood, and D. A. Cheresh Cooperation between VEGF and {beta}3 integrin during cardiac vascular development Blood, March 1, 2007; 109(5): 1962 - 1970. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hirakawa, M. Oike, M. Watanabe, Y. Karashima, and Y. Ito Pivotal role of integrin {alpha}5{beta}1 in hypotonic stress-induced responses of human endothelium FASEB J, October 1, 2006; 20(12): 1992 - 1999. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. G. Lamping, W. Zheng, D. Xing, L. P. Christensen, J. Martins, and R. J. Tomanek Bradycardia Stimulates Vascular Growth During Gradual Coronary Occlusion Arterioscler. Thromb. Vasc. Biol., October 1, 2005; 25(10): 2122 - 2127. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. E. Vlahakis and R. D. Hubmayr Cellular Stress Failure in Ventilator-injured Lungs Am. J. Respir. Crit. Care Med., June 15, 2005; 171(12): 1328 - 1342. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. G. Lloyd, B. M. Prior, H. Li, H. T. Yang, and R. L. Terjung VEGF receptor antagonism blocks arteriogenesis, but only partially inhibits angiogenesis, in skeletal muscle of exercise-trained rats Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H759 - H768. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Radel and V. Rizzo Integrin mechanotransduction stimulates caveolin-1 phosphorylation and recruitment of Csk to mediate actin reorganization Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H936 - H945. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. M. Prior, P. G. Lloyd, J. Ren, H. Li, H. T. Yang, M. H. Laughlin, and R. L. Terjung Time course of changes in collateral blood flow and isolated vessel size and gene expression after femoral artery occlusion in rats Am J Physiol Heart Circ Physiol, December 1, 2004; 287(6): H2434 - H2447. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang, J. Chang, Y.-C. Li, Y.-S. Li, J. Y.-J. Shyy, and S. Chien Shear stress and VEGF activate IKK via the Flk-1/Cbl/Akt signaling pathway Am J Physiol Heart Circ Physiol, February 1, 2004; 286(2): H685 - H692. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Geimonen, I. Fernandez, I. N. Gavrilovskaya, and E. R. Mackow Tyrosine Residues Direct the Ubiquitination and Degradation of the NY-1 Hantavirus G1 Cytoplasmic Tail J. Virol., October 15, 2003; 77(20): 10760 - 10868. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z.-G. Jin, H. Ueba, T. Tanimoto, A. O. Lungu, M. D. Frame, and B. C. Berk Ligand-Independent Activation of Vascular Endothelial Growth Factor Receptor 2 by Fluid Shear Stress Regulates Activation of Endothelial Nitric Oxide Synthase Circ. Res., August 22, 2003; 93(4): 354 - 363. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Duval, S. Bedard-Goulet, C. Delisle, and J.-P. Gratton Vascular Endothelial Growth Factor-dependent Down-regulation of Flk-1/KDR Involves Cbl-mediated Ubiquitination: CONSEQUENCES ON NITRIC OXIDE PRODUCTION FROM ENDOTHELIAL CELLS J. Biol. Chem., May 23, 2003; 278(22): 20091 - 20097. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
