Am J Physiol Cell Physiol AJP: Gastrointestinal and Liver Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 283: C1522-C1529, 2002. First published July 24, 2002; doi:10.1152/ajpcell.00115.2002
0363-6143/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/5/C1522    most recent
00115.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (33)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sterling, D.
Right arrow Articles by Casey, J. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sterling, D.
Right arrow Articles by Casey, J. R.
Vol. 283, Issue 5, C1522-C1529, November 2002

The functional and physical relationship between the DRA bicarbonate transporter and carbonic anhydrase II

Deborah Sterling1, Nathan J. D. Brown1, Claudiu T. Supuran2, and Joseph R. Casey1

1 Canadian Institutes of Health Research Membrane Protein Research Group, Departments of Physiology and Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7; and 2 Laboratorio di Chimca Bioinorganica, Dipartimento di Chemica, Università di Firenze, I-50019 Sesto Fiorentino, Florence, Italy

COOH-terminal cytoplasmic tails of chloride/bicarbonate anion exchangers (AE) bind cytosolic carbonic anhydrase II (CAII) to form a bicarbonate transport metabolon, a membrane protein complex that accelerates transmembrane bicarbonate flux. To determine whether interaction with CAII affects the downregulated in adenoma (DRA) chloride/bicarbonate exchanger, anion exchange activity of DRA-transfected HEK-293 cells was monitored by following changes in intracellular pH associated with bicarbonate transport. DRA-mediated bicarbonate transport activity of 18 ± 1 mM H+ equivalents/min was inhibited 53 ± 2% by 100 mM of the CAII inhibitor, acetazolamide, but was unaffected by the membrane-impermeant carbonic anhydrase inhibitor, 1-[5-sulfamoyl-1,3,4-thiadiazol-2-yl-(aminosulfonyl-4-phenyl)]-2,6-dimethyl-4-phenyl-pyridinium perchlorate. Compared with AE1, the COOH-terminal tail of DRA interacted weakly with CAII. Overexpression of a functionally inactive CAII mutant, V143Y, reduced AE1 transport activity by 61 ± 4% without effect on DRA transport activity (105 ± 7% transport activity relative to DRA alone). We conclude that cytosolic CAII is required for full DRA-mediated bicarbonate transport. However, DRA differs from other bicarbonate transport proteins because its transport activity is not stimulated by direct interaction with CAII.

metabolon; chloride/bicarbonate exchanger; downregulated in adenoma


This article has been cited by other articles:


Home page
 IOVSHome page
X. C. Sun, J. Li, M. Cui, and J. A. Bonanno
Role of Carbonic Anhydrase IV in Corneal Endothelial HCO3- Transport
Invest. Ophthalmol. Vis. Sci., March 1, 2008; 49(3): 1048 - 1055.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Gonzalez-Begne, T. Nakamoto, H.-V. Nguyen, A. K. Stewart, S. L. Alper, and J. E. Melvin
Enhanced Formation of a Formula Transport Metabolon in Exocrine Cells of Nhe1 / Mice
J. Biol. Chem., November 30, 2007; 282(48): 35125 - 35132.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
P. E. Morgan, S. Pastorekova, A. K. Stuart-Tilley, S. L. Alper, and J. R. Casey
Interactions of transmembrane carbonic anhydrase, CAIX, with bicarbonate transporters
Am J Physiol Cell Physiol, August 1, 2007; 293(2): C738 - C748.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. E. Simpson, C. W. Schweinfest, G. E. Shull, L. R. Gawenis, N. M. Walker, K. T. Boyle, M. Soleimani, and L. L. Clarke
PAT-1 (Slc26a6) is the predominant apical membrane Cl-/HCO3- exchanger in the upper villous epithelium of the murine duodenum
Am J Physiol Gastrointest Liver Physiol, April 1, 2007; 292(4): G1079 - G1088.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. Grosell and J. Genz
Ouabain-sensitive bicarbonate secretion and acid absorption by the marine teleost fish intestine play a role in osmoregulation
Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2006; 291(4): R1145 - R1156.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. Vidyasagar, V. M. Rajendran, and H. J. Binder
Three distinct mechanisms of HCO3- secretion in rat distal colon
Am J Physiol Cell Physiol, September 1, 2004; 287(3): C612 - C621.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. Krishnan, V. M. Rajendran, and H. J. Binder
Apical NHE isoforms differentially regulate butyrate-stimulated Na absorption in rat distal colon
Am J Physiol Cell Physiol, November 1, 2003; 285(5): C1246 - C1254.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online