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Am J Physiol Cell Physiol 283: C1511-C1521, 2002; doi:10.1152/ajpcell.01343.2000
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Vol. 283, Issue 5, C1511-C1521, November 2002

Dependence of Na+-K+ pump current-voltage relationship on intracellular Na+, K+, and Cs+ in rabbit cardiac myocytes

Peter S. Hansen1,2, Kerrie A. Buhagiar1,2, Benjamin Y. Kong3, Ronald J. Clarke3, David F. Gray1, and Helge H. Rasmussen1,2

1 Department of Cardiology, Royal North Shore Hospital, St. Leonards, NSW 2065; and 2 Department of Medicine and 3 School of Chemistry, University of Sydney, Sydney 2006, Australia

To examine effects of cytosolic Na+, K+, and Cs+ on the voltage dependence of the Na+-K+ pump, we measured Na+-K+ pump current (Ip) of ventricular myocytes voltage-clamped at potentials (Vm) from -100 to +60 mV. Superfusates were designed to eliminate voltage dependence at extracellular pump sites. The cytosolic compartment of myocytes was perfused with patch pipette solutions with a Na+ concentration ([Na]pip) of 80 mM and a K+ concentration from 0 to 80 mM or with solutions containing Na+ in concentrations from 0.1 to 100 mM and K+ in a concentration of either 0 or 80 mM. When [Na]pip was 80 mM, K+ in pipette solutions had a voltage-dependent inhibitory effect on Ip and induced a negative slope of the Ip-Vm relationship. Cs+ in pipette solutions had an effect on Ip qualitatively similar to that of K+. Increases in Ip with increases in [Na]pip were voltage dependent. The dielectric coefficient derived from [Na]pip-Ip relationships at the different test potentials was 0.15 when pipette solutions included 80 mM K+ and 0.06 when pipette solutions were K+ free.

access channels; ion transport; membrane dielectric; sodium/potassium-exchanging adenosinetriphosphatase


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