Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 283: C1422-C1431, 2002; doi:10.1152/ajpcell.00130.2002
0363-6143/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (39)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lytle, C.
Right arrow Articles by McManus, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lytle, C.
Right arrow Articles by McManus, T.
Vol. 283, Issue 5, C1422-C1431, November 2002

Coordinate modulation of Na-K-2Cl cotransport and K-Cl cotransport by cell volume and chloride

Christian Lytle1 and Thomas McManus2

1 Division of Biomedical Sciences, University of California, Riverside, California 92521; and 2 Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710

Na-K-2Cl cotransporter (NKCC) and K-Cl cotransporter (KCC) play key roles in cell volume regulation and epithelial Cl- transport. Reductions in either cell volume or cytosolic Cl- concentration ([Cl-]i) stimulate a corrective uptake of KCl and water via NKCC, whereas cell swelling triggers KCl loss via KCC. The dependence of these transporters on volume and [Cl-]i was evaluated in model duck red blood cells. Replacement of [Cl-]i with methanesulfonate elevated the volume set point at which NKCC activates and KCC inactivates. The set point was insensitive to cytosolic ionic strength. Reducing [Cl-]i at a constant driving force for inward NKCC and outward KCC caused the cells to adopt the new set point volume. Phosphopeptide maps of NKCC indicated that activation by cell shrinkage or low [Cl-]i is associated with phosphorylation of a similar constellation of Ser/Thr sites. Like shrinkage, reduction of [Cl-]i accelerated NKCC phosphorylation after abrupt inhibition of the deactivating phosphatase with calyculin A in vivo, whereas [Cl-] had no specific effect on dephosphorylation in vitro. Our results indicate that NKCC and KCC are reciprocally regulated by a negative feedback system dually modulated by cell volume and [Cl-]. The major effect of Cl- on NKCC is exerted through the volume-sensitive kinase that phosphorylates the transport protein.

sodium-potassium-chloride cotransport; intracellular chloride; cell volume regulation; ionic strength; cell water content; sodium-potassium-chloride cotransporter phosphorylation


This article has been cited by other articles:


Home page
 J. Neurophysiol.Home page
H. I. Rocha-Gonzalez, S. Mao, and F. J. Alvarez-Leefmans
Na+,K+,2Cl- Cotransport and Intracellular Chloride Regulation in Rat Primary Sensory Neurons: Thermodynamic and Kinetic Aspects
J Neurophysiol, July 1, 2008; 100(1): 169 - 184.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Physiol.Home page
M. L. Jennings and J. Cui
Chloride Homeostasis in Saccharomyces cerevisiae: High Affinity Influx, V-ATPase-dependent Sequestration, and Identification of a Candidate Cl- Sensor
J. Gen. Physiol., March 31, 2008; 131(4): 379 - 391.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
P. K. Lauf, S. Misri, A. A. Chimote, and N. C. Adragna
Apparent intermediate K conductance channel hyposmotic activation in human lens epithelial cells
Am J Physiol Cell Physiol, March 1, 2008; 294(3): C820 - C832.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
D. K. Nielsen, A. K. Jensen, H. Harbak, S. C. Christensen, and L. O. Simonsen
Cell content of phosphatidylinositol (4,5)bisphosphate in Ehrlich mouse ascites tumour cells in response to cell volume perturbations in anisotonic and in isosmotic media
J. Physiol., August 1, 2007; 582(3): 1027 - 1036.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
K. T. Kahle, J. Rinehart, A. Ring, I. Gimenez, G. Gamba, S. C. Hebert, and R. P. Lifton
WNK Protein Kinases Modulate Cellular Cl- Flux by Altering the Phosphorylation State of the Na-K-Cl and K-Cl Cotransporters.
Physiology, October 1, 2006; 21: 326 - 335.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
K. Strange, J. Denton, and K. Nehrke
Ste20-Type Kinases: Evolutionarily Conserved Regulators of Ion Transport and Cell Volume
Physiology, February 1, 2006; 21(1): 61 - 68.
[Abstract] [Full Text] [PDF]

Home page
 Exp PhysiolHome page
K. J Treharne, R. M Crawford, and A Mehta
CFTR, chloride concentration and cell volume: could mammalian protein histidine phosphorylation play a latent role?
Exp Physiol, January 1, 2006; 91(1): 131 - 139.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. D. Ciano-Oliveira, M. Lodyga, L. Fan, K. Szaszi, H. Hosoya, O. D. Rotstein, and A. Kapus
Is myosin light-chain phosphorylation a regulatory signal for the osmotic activation of the Na+-K+-2Cl- cotransporter?
Am J Physiol Cell Physiol, July 1, 2005; 289(1): C68 - C81.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. L. Kreindler, A. D. Jackson, P. A. Kemp, R. J. Bridges, and H. Danahay
Inhibition of chloride secretion in human bronchial epithelial cells by cigarette smoke extract
Am J Physiol Lung Cell Mol Physiol, May 1, 2005; 288(5): L894 - L902.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Physiol.Home page
J. Denton, K. Nehrke, X. Yin, R. Morrison, and K. Strange
GCK-3, a Newly Identified Ste20 Kinase, Binds To and Regulates the Activity of a Cell Cycle-dependent ClC Anion Channel
J. Gen. Physiol., January 31, 2005; 125(2): 113 - 125.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. R. Williams and J. A. Payne
Cation transport by the neuronal K+-Cl- cotransporter KCC2: thermodynamics and kinetics of alternate transport modes
Am J Physiol Cell Physiol, October 1, 2004; 287(4): C919 - C931.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. F. X. Dowd and B. Forbush
PASK (Proline-Alanine-rich STE20-related Kinase), a Regulatory Kinase of the Na-K-Cl Cotransporter (NKCC1)
J. Biol. Chem., July 18, 2003; 278(30): 27347 - 27353.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online