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Am J Physiol Cell Physiol 283: C1080-C1089, 2002. First published May 29, 2002; doi:10.1152/ajpcell.00048.2002
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Vol. 283, Issue 4, C1080-C1089, October 2002

NO upregulation of a cyclic nucleotide-gated channel contributes to calcium elevation in endothelial cells

Jianliang Zhang1, Shen-Ling Xia1,2, Edward R. Block1,2, and Jawaharlal M. Patel1,2

1 Department of Medicine, University of Florida, and 2 Medical Research Service, Malcom Randall Veterans Affairs Medical Center, Gainesville, Florida 32608-1197

We investigated whether nitric oxide (NO) upregulates a cyclic nucleotide-gated (CNG) channel and whether this contributes to sustained elevation of intracellular calcium levels ([Ca2+]i) in porcine pulmonary artery endothelial cells (PAEC). Exposure of PAEC to an NO donor, NOC-18 (1 mM), for 18 h increased the protein and mRNA levels of CNGA2 40 and 50%, respectively (P < 0.05). [Ca2+]i in NO-treated cells was increased 50%, and this increase was maintained for up to 12 h after removal of NOC-18 from medium. Extracellular calcium is required for the increase in [Ca2+]i in NO-treated cells. Thapsigargin induced a rapid cytosolic calcium rise, whereas both a CNG and a nonselective cation channel blocker caused a faster decline in [Ca2+]i, suggesting that capacitive calcium entry contributes to the elevated calcium levels. Antisense inhibition of CNGA2 expression attenuated the NO-induced increases in CNGA2 expression and [Ca2+]i and in capacitive calcium entry. Our results demonstrate that exogenous NO upregulates CNGA2 expression and that this is associated with elevated [Ca2+]i and capacitive calcium entry in porcine PAEC.

nitric oxide; lung; gene; upregulation


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