Am J Physiol Cell Physiol AJP citation statistics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 283: C885-C894, 2002. First published May 15, 2002; doi:10.1152/ajpcell.00085.2002
0363-6143/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/3/C885    most recent
00085.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (15)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sergeant, G. P.
Right arrow Articles by Hollywood, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sergeant, G. P.
Right arrow Articles by Hollywood, M. A.
Vol. 283, Issue 3, C885-C894, September 2002

Characterization of norepinephrine-evoked inward currents in interstitial cells isolated from the rabbit urethra

G. P. Sergeant, K. D. Thornbury, N. G. McHale, and M. A. Hollywood

Smooth Muscle Group, Department of Physiology, The Queen's University of Belfast, BT9 7BL Belfast, United Kingdom

Freshly dispersed interstitial cells from the rabbit urethra were studied by using the perforated-patch technique. When cells were voltage clamped at -60 mV and exposed to 10 µM norepinephrine (NE) at 80-s intervals, either large single inward currents or a series of oscillatory inward currents of diminishing amplitude were evoked. These currents were blocked by either phentolamine (1 µM) or prazosin (1 µM), suggesting that the effects of NE were mediated via alpha 1-adrenoceptors. NE-evoked currents were depressed by the blockers of Ca2+-activated Cl- currents, niflumic acid (10 µM), and 9-anthracenecarboxylic acid (9-AC, 1 mM). The reversal potential of the above currents changed in a predictable manner when the Cl- equilibrium potential was altered, again suggesting that they were due to activation of a Cl- conductance. NE-evoked currents were decreased by 10 µM cyclopiazonic acid, suggesting that they were dependent on store-released Ca2+. Inhibition of NE-evoked currents by the phospholipase C inhibitor 2-nitro-4-carboxyphenyl-N,N-diphenylcarbamate (100 µM) suggested that NE releases Ca2+ via an inositol 1,4,5-trisphosphate (IP3)-dependent mechanism. These results support the idea that stimulation of alpha 1-adrenoceptors releases Ca2+ from an IP3-sensitive store, which in turn activates Ca2+-activated Cl- current in freshly dispersed interstitial cells of the rabbit urethra. This elevates slow wave frequency in these cells and may underlie the mechanism responsible for increased urethral tone during nerve stimulation.

chloride; pacemaker cell; neurotransmission


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
D. Triguero, M. Sancho, M. Garcia-Flores, and A. Garcia-Pascual
Presence of cyclic nucleotide-gated channels in the rat urethra and their involvement in nerve-mediated nitrergic relaxation
Am J Physiol Renal Physiol, November 1, 2009; 297(5): F1353 - F1360.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
A. Garcia-Pascual, M. Sancho, G. Costa, and D. Triguero
Interstitial cells of Cajal in the urethra are cGMP-mediated targets of nitrergic neurotransmission
Am J Physiol Renal Physiol, October 1, 2008; 295(4): F971 - F983.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
G. P. Sergeant, E. Bradley, K. D. Thornbury, N. G. McHale, and M. A. Hollywood
Role of mitochondria in modulation of spontaneous Ca2+ waves in freshly dispersed interstitial cells of Cajal from the rabbit urethra
J. Physiol., October 1, 2008; 586(19): 4631 - 4642.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
H. Hashitani and H. Suzuki
Properties of spontaneous Ca2+ transients recorded from interstitial cells of Cajal-like cells of the rabbit urethra in situ
J. Physiol., September 1, 2007; 583(2): 505 - 519.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
G. P. Sergeant, L. Johnston, N. G. McHale, K. D. Thornbury, and M. A. Hollywood
Activation of the cGMP/PKG pathway inhibits electrical activity in rabbit urethral interstitial cells of Cajal by reducing the spatial spread of Ca2+ waves
J. Physiol., July 1, 2006; 574(1): 167 - 181.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
E. Bradley, M. A. Hollywood, N. G. McHale, K. D. Thornbury, and G. P. Sergeant
Pacemaker activity in urethral interstitial cells is not dependent on capacitative calcium entry
Am J Physiol Cell Physiol, September 1, 2005; 289(3): C625 - C632.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
L. Johnston, G. P. Sergeant, M. A. Hollywood, K. D. Thornbury, and N. G. McHale
Calcium oscillations in interstitial cells of the rabbit urethra
J. Physiol., June 1, 2005; 565(2): 449 - 461.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
K.-E. Andersson and A. J. Wein
Pharmacology of the Lower Urinary Tract: Basis for Current and Future Treatments of Urinary Incontinence
Pharmacol. Rev., December 1, 2004; 56(4): 581 - 631.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
H Suzuki, S M Ward, Y R Bayguinov, F R Edwards, and G D S Hirst
Involvement of intramuscular interstitial cells in nitrergic inhibition in the mouse gastric antrum
J. Physiol., February 1, 2003; 546(3): 751 - 763.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online