Am J Physiol Cell Physiol Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 283: C822-C830, 2002. First published April 24, 2002; doi:10.1152/ajpcell.00545.2001
0363-6143/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/3/C822    most recent
00545.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (7)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Inuyama, M.
Right arrow Articles by Sawada, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Inuyama, M.
Right arrow Articles by Sawada, Y.
Vol. 283, Issue 3, C822-C830, September 2002

Characteristics of L-lactic acid transport in basal membrane vesicles of human placental syncytiotrophoblast

Masako Inuyama1, Fumihiko Ushigome1, Akiko Emoto1, Noriko Koyabu1, Shoji Satoh2, Kiyomi Tsukimori2, Hitoo Nakano2, Hisakazu Ohtani1, and Yasufumi Sawada1

1 Department of Medico-Pharmaceutical Sciences, Graduate School of Pharmaceutical Sciences, and 2 Department of Reproduction and Gynecology, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8582, Japan

The characteristics of L-lactic acid transport across the trophoblast basal membrane were investigated and compared with those across the brush-border membrane by using membrane vesicles isolated from human placenta. The uptake of L-[14C]lactic acid into basal membrane vesicles was Na+ independent, and an uphill transport was observed in the presence of a pH gradient ([H+]out > [H+]in). L-[14C]lactic acid uptake exhibited saturation kinetics with a Km value of 5.89 ± 0.68 mM in the presence of a pH gradient. p-Chloromercuribenzenesulfonate and alpha -cyano-4-hydroxycinnamate inhibited the initial uptake, whereas phloretin or 4,4'-diisothiocyanostilbene-2,2'-disulfonate did not. Mono- and dicarboxylic acids suppressed the initial uptake. In conclusion, L-lactic acid transport in the basal membrane is H+ dependent and Na+ independent, as is also the case for the brush-border membrane transport, and its characteristics resemble those of monocarboxylic acid transporters. However, there were several differences in the effects of inhibitors between basal and brush-border membrane vesicles, suggesting that the transporter(s) involved in L-lactic acid transport in the basal membrane of placental trophoblast may differ from those in the brush-border membrane.

human placenta; trophoblast; monocarboxylic acid


This article has been cited by other articles:


Home page
 Reproductive SciencesHome page
P. Settle, C. P. Sibley, I. M. Doughty, T. Johnston, J. D. Glazier, T. L. Powell, T. Jansson, and S. W. D'Souza
Placental Lactate Transporter Activity and Expression in Intrauterine Growth Restriction
Reproductive Sciences, July 1, 2006; 13(5): 357 - 363.
[Abstract] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
K. Lahjouji, I. Elimrani, J. Lafond, L. Leduc, I. A. Qureshi, and G. A. Mitchell
L-Carnitine transport in human placental brush-border membranes is mediated by the sodium-dependent organic cation transporter OCTN2
Am J Physiol Cell Physiol, August 1, 2004; 287(2): C263 - C269.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
R. Mengual, K. el Abida, N. Mouaffak, M. Rieu, and M. Beaudry
Pyruvate shuttle in muscle cells: high-affinity pyruvate transport sites insensitive to trans-lactate efflux
Am J Physiol Endocrinol Metab, December 1, 2003; 285(6): E1196 - E1204.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online