Am J Physiol Cell Physiol Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 283: C752-C761, 2002. First published May 8, 2002; doi:10.1152/ajpcell.00501.2001
0363-6143/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/3/C752    most recent
00501.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (11)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Komatsu, S.
Right arrow Articles by Ikebe, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Komatsu, S.
Right arrow Articles by Ikebe, M.
Vol. 283, Issue 3, C752-C761, September 2002

Translocation of telokin by cGMP signaling in smooth muscle cells

Satoshi Komatsu1, Koji Miyazaki1, Richard A. Tuft2, and Mitsuo Ikebe1

1 Department of Physiology and 2 Biomedical Imaging Group, University of Massachusetts Medical School, Worcester, Massachusetts 01655

Telokin is an acidic protein with a sequence identical to the COOH-terminal domain of myosin light chain kinase (MLCK) produced by an alternate promoter of the MLCK gene. Although it is abundantly expressed in smooth muscle, its physiological function is not understood. In the present study, we attempted to clarify the function of telokin by analyzing its spatial and temporal localization in living single smooth muscle cells. Primary cultured smooth muscle cells were transfected with green fluorescent protein (GFP)-tagged telokin. The telokin-GFP localized mostly diffusely in cytosol. Stimulation with both sodium nitroprusside (SNP) and 8-bromo-cyclic GMP induced translocation of GFP-tagged telokin to near plasma membrane in living single smooth muscle cells. The translocation was slow, and it took more than 10 min at room temperature. Mutation of the phosphorylation sites of telokin (S13A, S19A, and S13A/S19A) significantly attenuated SNP-induced translocation. Both KT-5823 (cGMP-dependent protein kinase inhibitor) and PD-98059 (mitogen-activated protein kinase inhibitor) diminished the telokin-GFP translocation. These results suggest that telokin changes its intracellular localization because of phosphorylation at Ser13 and/or Ser19 via the cGMP-signaling pathway.

green fluorescent protein; myosin; myosin light chain kinase; guanosine 3',5'-cyclic monophosphate-dependent protein kinase; phosphorylation


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. A. Madden, M. W. Dantuma, E. A. Sorokina, D. Weihrauch, and J. G. Kleinman
Telokin expression and the effect of hypoxia on its phosphorylation status in smooth muscle cells from small and large pulmonary arteries
Am J Physiol Lung Cell Mol Physiol, June 1, 2008; 294(6): L1166 - L1173.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
Y. Koga and M. Ikebe
A Novel Regulatory Mechanism of Myosin Light Chain Phosphorylation via Binding of 14-3-3 to Myosin Phosphatase
Mol. Biol. Cell, March 1, 2008; 19(3): 1062 - 1071.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. Komatsu and M. Ikebe
The Phosphorylation of Myosin II at the Ser1 and Ser2 Is Critical for Normal Platelet-derived Growth Factor induced Reorganization of Myosin Filaments
Mol. Biol. Cell, December 1, 2007; 18(12): 5081 - 5090.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
B. P. Herring, O. El-Mounayri, P. J. Gallagher, F. Yin, and J. Zhou
Regulation of myosin light chain kinase and telokin expression in smooth muscle tissues
Am J Physiol Cell Physiol, November 1, 2006; 291(5): C817 - C827.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
L. Deak, J. Zheng, A. Orem, G.-G. Du, S. Aguinaga, K. Matsuda, and P. Dallos
Effects of cyclic nucleotides on the function of prestin
J. Physiol., March 1, 2005; 563(2): 483 - 496.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
A. P. SOMLYO and A. V. SOMLYO
Ca2+ Sensitivity of Smooth Muscle and Nonmuscle Myosin II: Modulated by G Proteins, Kinases, and Myosin Phosphatase
Physiol Rev, October 1, 2003; 83(4): 1325 - 1358.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online