Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 283: C631-C638, 2002. First published April 18, 2002; doi:10.1152/ajpcell.00248.2001
0363-6143/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/2/C631    most recent
00248.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (8)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fanzo, J. C.
Right arrow Articles by Lei, K. Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fanzo, J. C.
Right arrow Articles by Lei, K. Y.
Vol. 283, Issue 2, C631-C638, August 2002

p53 protein and p21 mRNA levels and caspase-3 activity are altered by zinc status in aortic endothelial cells

Jessica C. Fanzo1, Scott K. Reaves1, Libin Cui2, Lei Zhu2, and Kai Y. Lei2

1 Department of Nutritional Sciences, University of Arizona, Tucson, Arizona 85721; and 2 Department of Nutrition and Food Science, University of Maryland, College Park, Maryland 20742

The influence of zinc status on the levels of p53, as well as downstream targets of p53 in cell repair and survival, was examined in human aortic endothelial cells (HAECs). A serum-reduced low-zinc medium (ZD) was used to deplete zinc over one passage. Other treatments included zinc-normal control (ZN), zinc-adequate (ZA), and zinc-supplemented (ZS) treatment with 3.0, 16.0, and 32.0 µM zinc, respectively. Cellular zinc levels in the ZD cells were 64% of ZN controls; levels in the ZA cells were not different, but levels in ZS cells were significantly higher (40%) than in ZN cells. No difference in p53 mRNA abundance was detected among all treatments; however, p53 nuclear protein levels were >100% higher in the ZD and ZS cells and almost 200% higher in the ZA cells than in ZN controls. In addition, p21 mRNA abundance, a downstream target of p53 protein, was increased in the ZS cells compared with both the ZN control and ZD cells. In the ZS cells, bax and mcl-1 were also ~50% higher compared with ZN controls, whereas bcl-2 mRNA was increased compared with ZA cells. Moreover, caspase-3 activity of ZD cells was not different from that of ZN controls but was reduced to 83 and 69% of ZN controls in ZA and ZS cells, respectively. Thus p53 protein and p53 downstream target genes appeared to be modulated by intracellular zinc status in HAECs.

p53 tumor suppressor gene; apoptosis; zinc depleted; zinc supplemented; atherosclerosis


This article has been cited by other articles:


Home page
 J. Nutr.Home page
M. Yan, Y. Song, C. P. Wong, K. Hardin, and E. Ho
Zinc Deficiency Alters DNA Damage Response Genes in Normal Human Prostate Epithelial Cells
J. Nutr., April 1, 2008; 138(4): 667 - 673.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. H. K. Wong, Y. Zhao, N. W. Schoene, C.-T. Han, R. S. M. Shih, and K. Y. Lei
Zinc deficiency depresses p21 gene expression: inhibition of cell cycle progression is independent of the decrease in p21 protein level in HepG2 cells
Am J Physiol Cell Physiol, June 1, 2007; 292(6): C2175 - C2184.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
A. A. Alshatwi, C.-T. Han, N. W. Schoene, and K. Y. Lei
Nuclear Accumulations of p53 and Mdm2 Are Accompanied by Reductions in c-Abl and p300 in Zinc-Depleted Human Hepatoblastoma Cells.
Experimental Biology and Medicine, May 1, 2006; 231(5): 611 - 618.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
E. Ho, C. Courtemanche, and B. N. Ames
Zinc Deficiency Induces Oxidative DNA Damage and Increases P53 Expression in Human Lung Fibroblasts
J. Nutr., August 1, 2003; 133(8): 2543 - 2548.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online