Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 283: C599-C608, 2002. First published April 24, 2002; doi:10.1152/ajpcell.00065.2002
0363-6143/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/2/C599    most recent
00065.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (9)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Koumas, L.
Right arrow Articles by Phipps, R. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Koumas, L.
Right arrow Articles by Phipps, R. P.
Vol. 283, Issue 2, C599-C608, August 2002

Differential COX localization and PG release in Thy-1+ and Thy-1minus human female reproductive tract fibroblasts

Laura Koumas1 and Richard P. Phipps1,2,3

Departments of 1 Microbiology and Immunology, 2 Environmental Medicine, and 3 Pediatrics and Obstetrics and Gynecology, University of Rochester School of Medicine and Dentistry, Lung Biology and Disease Program, Rochester, New York 14642

A key role exists for prostaglandins (PGs) in reproductive health, including fertility and parturition. However, the cellular sources and regulation of PG production by cyclooxygenase (COX) in the human female reproductive tract remain poorly understood. We recently reported that human female reproductive tract fibroblasts are divisible into distinct subsets based on their Thy-1 surface expression. Herein, we demonstrate that the expression, induction, and subcellular localization of COX-1 and COX-2 and the downstream PG biosynthesis are markedly different between these subsets. Specifically, Thy-1+ fibroblasts highly express COX-1, which is responsible for high-level PGE2 production, a feature usually attributed to the COX-2 isoenzyme. In contrast, COX-2, generally considered an inducible isoform, is constitutively expressed in the Thy-1- subset, which only minimally produces PGE2. The intracellular signaling pathways for COX regulation also differ between the subsets. Determination of differences in signal transduction, COX expression and localization, and PG production by human reproductive fibroblast subtypes supports the concept of fibroblast heterogeneity and the possibility that these subsets may play unique roles in tissue homeostasis and in inflammation.

inflammation; myometrium; lipid mediators; heterogeneity; prostaglandin; cyclooxygenase


This article has been cited by other articles:


Home page
 Mol Hum ReprodHome page
M. Zaitseva, B. J. Vollenhoven, and P. A.W. Rogers
Retinoic acid pathway genes show significantly altered expression in uterine fibroids when compared with normal myometrium
Mol. Hum. Reprod., August 1, 2007; 13(8): 577 - 585.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. A. Martey, C. J. Baglole, T. A. Gasiewicz, P. J. Sime, and R. P. Phipps
The aryl hydrocarbon receptor is a regulator of cigarette smoke induction of the cyclooxygenase and prostaglandin pathways in human lung fibroblasts
Am J Physiol Lung Cell Mol Physiol, September 1, 2005; 289(3): L391 - L399.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
G. Ishii, T. Sangai, K. Sugiyama, T. Ito, T. Hasebe, Y. Endoh, J. Magae, and A. Ochiai
In Vivo Characterization of Bone Marrow-Derived Fibroblasts Recruited into Fibrotic Lesions
Stem Cells, May 1, 2005; 23(5): 699 - 706.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
J. C. Havelock, P. Keller, N. Muleba, B. A. Mayhew, B. M. Casey, W. E. Rainey, and R. A. Word
Human Myometrial Gene Expression Before and During Parturition
Biol Reprod, March 1, 2005; 72(3): 707 - 719.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. A. Martey, S. J. Pollock, C. K. Turner, K. M. A. O'Reilly, C. J. Baglole, R. P. Phipps, and P. J. Sime
Cigarette smoke induces cyclooxygenase-2 and microsomal prostaglandin E2 synthase in human lung fibroblasts: implications for lung inflammation and cancer
Am J Physiol Lung Cell Mol Physiol, November 1, 2004; 287(5): L981 - L991.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online