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Am J Physiol Cell Physiol 283: C77-C84, 2002. First published February 13, 2002; doi:10.1152/ajpcell.00456.2001
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Vol. 283, Issue 1, C77-C84, July 2002

Pore formation is not associated with macroscopic redistribution of P2X7 receptors

Megan L. Smart1,2, Rekha G. Panchal1,2, David N. Bowser1,2, David A. Williams2, and Steven Petrou1

1 The Laboratory of Biophysics and Molecular Physiology and 2 The Laboratory of Confocal and Fluorescence Imaging, Department of Physiology, The University of Melbourne, Victoria 3010, Australia

The present study examines whether changes in P2X7 purinergic receptor density precede formation of the cytolytic pore characteristic of this receptor. We fused P2X7 receptors with enhanced green fluorescent protein (EGFP) at the amino or carboxy termini (EGFP-P2X7 and P2X7-EGFP). Electrophysiological characterization in Xenopus oocytes revealed wild-type responses to ATP for GFP-tagged receptors. However, differences in sensitivity to ATP were apparent with the P2X7-EGFP receptor displaying a threefold reduction in ATP sensitivity compared with control. Ethidium ion uptake was used to measure cytolytic pore formation. Comparison of tagged receptors with wild type in HEK-293 and COS-7 cells showed there was no significant difference in ethidium ion uptake, suggesting that fusions with EGFP did not interfere with cytolytic pore formation. Confocal microscopy confirmed that tagged receptors localized to the plasmalemma. Simultaneous monitoring of EGFP and ethidium ion fluorescence revealed that changes in receptor distribution do not precede pore formation. We conclude that it is unlikely that large scale changes in P2X7 receptor density precede pore formation.

pore-forming protein; cytolytic; HEK-293; P2Z; enhanced green fluorescent protein; fusion protein


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