Am J Physiol Cell Physiol Watch the video to see how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 283: C204-C211, 2002. First published March 6, 2002; doi:10.1152/ajpcell.00574.2001
0363-6143/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/1/C204    most recent
00574.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (25)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Spangenburg, E. E.
Right arrow Articles by Booth, F. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Spangenburg, E. E.
Right arrow Articles by Booth, F. W.
Vol. 283, Issue 1, C204-C211, July 2002

Multiple signaling pathways mediate LIF-induced skeletal muscle satellite cell proliferation

Espen E. Spangenburg and Frank W. Booth

Department of Biomedical Sciences, College of Veterinary Medicine, University of Missouri-Columbia, Columbia, Missouri 65211

There are many known growth factors/cytokines that induce skeletal muscle satellite cell proliferation. Currently, the signaling mechanisms in which these growth factors/cytokines activate satellite cell proliferation are not completely understood. Here, we sought to determine signaling mechanisms by which leukemia inhibitory factor (LIF) induces satellite cell proliferation in culture. First, we confirmed that LIF induces proliferation of C2C12 immortalized myoblasts and cultured primary rat satellite cells. In addition, we also found that this increase in proliferation can be inhibited by incubation of the cells in tyrphostin AG 490, a specific inhibitor of Janus-activated kinase (JAK) 2 activity. Furthermore, we also found that incubation of the cells at various time points with LIF (10 ng/ml) induces a significant, transient increase in JAK2 phosphorylation, signal transducers and activators of transcription (STAT3) phosphorylation, and STAT3 transcriptional activity. Increases in the STAT3-sensitive endogenous SOC3 protein followed these transient increases in STAT3 activation. In addition, AG 490 inhibited the increase in STAT3 phosphorylation. Finally, LIF did not change the phosphorylation status of extracellular signal-regulated protein kinase (ERK)1/2 or affect the phosphorylation status of Akt/protein kinase B. However, LY-294002, an inhibitor of phosphoinositide 3-kinase, blocked LIF-induced proliferation of satellite cells. These data suggest that LIF induces satellite cell proliferation by activation of the JAK2-STAT3 signaling pathway, suggesting that this may be an important pathway in muscle growth and/or hypertrophy.

cytokine; mitogen; Janus-activated kinase; leukemia inhibitory factor; signal transducers and activators of transcription


This article has been cited by other articles:


Home page
 J. Physiol.Home page
L. Yahiaoui, D. Gvozdic, G. Danialou, M. Mack, and B. J. Petrof
CC family chemokines directly regulate myoblast responses to skeletal muscle injury
J. Physiol., August 15, 2008; 586(16): 3991 - 4004.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
C. Broholm, O. H. Mortensen, S. Nielsen, T. Akerstrom, A. Zankari, B. Dahl, and B. K. Pedersen
Exercise induces expression of leukaemia inhibitory factor in human skeletal muscle
J. Physiol., April 15, 2008; 586(8): 2195 - 2201.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
L. Sun, K. Ma, H. Wang, F. Xiao, Y. Gao, W. Zhang, K. Wang, X. Gao, N. Ip, and Z. Wu
JAK1 STAT1 STAT3, a key pathway promoting proliferation and preventing premature differentiation of myoblasts
J. Cell Biol., October 8, 2007; 179(1): 129 - 138.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. K. Trenerry, K. A. Carey, A. C. Ward, and D. Cameron-Smith
STAT3 signaling is activated in human skeletal muscle following acute resistance exercise
J Appl Physiol, April 1, 2007; 102(4): 1483 - 1489.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
R. T. Morris, E. E. Spangenburg, and F. W. Booth
Responsiveness of cell signaling pathways during the failed 15-day regrowth of aged skeletal muscle
J Appl Physiol, January 1, 2004; 96(1): 398 - 404.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
S. B. P. CHARGE and M. A. RUDNICKI
Cellular and Molecular Regulation of Muscle Regeneration
Physiol Rev, January 1, 2004; 84(1): 209 - 238.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
T. E. Childs, E. E. Spangenburg, D. R. Vyas, and F. W. Booth
Temporal alterations in protein signaling cascades during recovery from muscle atrophy
Am J Physiol Cell Physiol, August 1, 2003; 285(2): C391 - C398.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
D. Metcalf
The Unsolved Enigmas of Leukemia Inhibitory Factor
Stem Cells, January 1, 2003; 21(1): 5 - 14.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online