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Am J Physiol Cell Physiol 282: C1396-C1403, 2002; doi:10.1152/ajpcell.00615.2001
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Vol. 282, Issue 6, C1396-C1403, June 2002

Inward rectifier K+ channel Kir2.3 is localized at the postsynaptic membrane of excitatory synapses

Atsushi Inanobe1, Akikazu Fujita1, Minoru Ito2, Hitonobu Tomoike3, Kiyoshi Inageda1, and Yoshihisa Kurachi1

1 Department of Pharmacology II, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871; 2 First Department of Internal Medicine, Yamagata University School of Medicine, Yamagata 990-9585; and 3 Hospital of National Cardiovascular Center, Suita, Osaka 565-8565, Japan

Classical inwardly rectifying K+ channels (Kir2.0) are responsible for maintaining the resting membrane potential near the K+ equilibrium potential in various cells, including neurons. Although Kir2.3 is known to be expressed abundantly in the forebrain, its precise localization has not been identified. Using an antibody specific to Kir2.3, we examined the subcellular localization of Kir2.3 in mouse brain. Kir2.3 immunoreactivity was detected in a granular pattern in restricted areas of the brain, including the olfactory bulb (OB). Immunoelectron microscopy of the OB revealed that Kir2.3 immunoreactivity was specifically clustered on the postsynaptic membrane of asymmetric synapses between granule cells and mitral/tufted cells. The immunoprecipitants for Kir2.3 obtained from brain contained PSD-95 and chapsyn-110, PDZ domain-containing anchoring proteins. In vitro binding assay further revealed that the COOH-terminal end of Kir2.3 is responsible for the association with these anchoring proteins. Therefore, the Kir channel may be involved in formation of the resting membrane potential of the spines and, thus, would affect the response of N-methyl-D-aspartic acid receptor channels at the excitatory postsynaptic membrane.

dendritic spine; ion channel localization; excitatory synaptic transmission


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