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Am J Physiol Cell Physiol 282: C1205-C1211, 2002. First published February 6, 2002; doi:10.1152/ajpcell.00511.2001
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Vol. 282, Issue 6, C1205-C1211, June 2002

Lipopolysaccharide stimulation of ERK1/2 increases TNF-alpha production via Egr-1

Liang Shi, Raj Kishore, Megan R. McMullen, and Laura E. Nagy3

Department of Nutrition, Case Western Reserve University, Cleveland, Ohio 44106-4906

Lipopolysaccharide (LPS) is a potent activator of tumor necrosis factor-alpha (TNF-alpha ) production by macrophages. LPS stimulates the phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 and increases TNF-alpha mRNA and protein accumulation in RAW 264.7 murine macrophages. However, the role of ERK1/2 activation in mediating LPS-stimulated TNF-alpha production is not well understood. Inhibition of ERK1/2 activation with PD-98059 or overexpression of dominant negative ERK1/2 decreased LPS-induced TNF-alpha mRNA quantity. LPS rapidly increased early growth response factor (Egr)-1 binding to the TNF-alpha promoter; this response was blunted in cells treated with PD-98059 or transfected with dominant-negative ERK1/2. Using a chloramphenicol acetyltransferase reporter gene linked to the Egr-1 promoter, we show that LPS increased Egr-1 promoter activity via an ERK1/2-dependent mechanism. These results delineate the role of ERK1/2 activation of Egr-1 activity in mediating LPS-induced increases in TNF-alpha mRNA expression in macrophages.

monocytes/macrophages; lipopolysaccharide; protein kinases/phosphatases; transcription factors; signal transduction; extracellular signal-regulated kinase 1/2; early growth response factor-1; tumor necrosis factor-alpha

3 Address for reprint requests and other correspondence: L. E. Nagy, Dept. of Nutrition, Case Western Reserve Univ., 2123 Abington Rd., Rm. 201, Cleveland, OH 44106-4906 (E-mail: len2{at}po.cwru.edu).




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