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Departamento de Fisiología, Facultad de Medicina, Universidad de Santiago de Compostela, San Francisco s/n, 15705 Santiago de Compostela, Spain
Myostatin (MSTN), a
transforming growth factor (TGF)-
superfamily member, has been shown
to negatively regulate muscle growth by inhibiting muscle precursor
cell proliferation. Here, we stably transfected
C2C12 cells with mouse MSTN cDNA to investigate
its possible role in myoblast differentiation. We found that MSTN cDNA
overexpression reversibly inhibits the myogenic process by downregulating mRNA levels of the muscle regulatory factors myoD and
myogenin, as well as the activity of their downstream target creatine
kinase. Taking into consideration that MSTN expression during
development is restricted to muscle, our results suggest that MSTN
probably regulates myogenic differentiation by an autocrine mechanism.
muscle differentiation; muscle regulatory factors; transforming
growth factor- superfamily
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