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Am J Physiol Cell Physiol 282: C973-C979, 2002. First published January 2, 2002; doi:10.1152/ajpcell.00415.2001
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Vol. 282, Issue 5, C973-C979, May 2002

TRANSLATIONAL PHYSIOLOGY
Regulation of transferrin-induced endocytosis by wild-type and C282Y-mutant HFE in transfected HeLa cells

Lukas Schwake1,*, Andreas W. Henkel2,*, Hans D. Riedel1, Thorsten Schlenker1, Matthias Both2, Andrea Migala2, Boris Hadaschik1, Nataly Henfling1, and Wolfgang Stremmel1

1 Department of Gastroenterology, Faculty of Medicine, University of Heidelberg, D-69115 Heidelberg; and 2 Department of Biomedical Optics, Max-Planck-Institute for Medical Research, D-69120 Heidelberg, Germany

The hereditary hemochromatosis protein HFE is known to complex with the transferrin receptor; however, its function regarding endocytosis of transferrin is unclear. We performed patch-clamp capacitance measurements in transfected HeLa cells carrying wild-type or C282Y-mutant HFE cDNA under the control of a tetracycline-sensitive promoter. Whole cell experiments in cells with suppressed expression of wild-type HFE revealed a decrease in membrane capacitance, reflecting predominance of endocytosis in the presence of transferrin. Cells overexpressing C282Y-mutant HFE displayed less intense capacitance decreases, whereas no significant decrease was observed in cells overexpressing wild-type HFE. The formation of single endocytic vesicles in cells with suppressed expression of wild-type HFE was greatly increased in the presence of transferrin as revealed by cell-attached recordings. According to their calculated diameters, many of these vesicles corresponded to clathrin-coated vesicles. These results suggest that wild-type HFE negatively modulates the endocytic uptake of transferrin. This inhibitory effect is attenuated in cells expressing C282Y-mutant HFE. Time-resolved measurements of cell membrane capacitance provide a powerful tool to study transferrin-induced endocytosis in single cells.

hereditary hemochromatosis; transferrin receptor; patch-clamp capacitance measurements

* L. Schwake and A. W. Henkel contributed equally to this work.




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