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Service de Biochimie, Faculté de Médecine Paris-Ouest, Université Descartes (Paris V), Centre hospitalier de Poissy, 78303 Poissy Cedex, France
Because leptin has recently been
shown to induce proliferation and/or differentiation of different cell
types through different pathways, the aim of the present study was to
investigate, in vitro, the influence of leptin on adipogenesis in rat
preadipocytes. A prerequisite to this study was to identify leptin
receptors (Ob-Ra and Ob-Rb) in preadipocytes from femoral subcutaneous
fat. We observed that expressions of Ob-Ra and Ob-Rb increase during adipogenesis. Furthermore, leptin induces an increase of p42/p44 mitogen-activated protein kinase phosphorylated isoforms in both confluent and differentiated preadipocytes and of STAT3 phosphorylation only in confluent preadipocytes. Moreover, exposure to leptin promoted
activator protein-1 complex DNA binding activity in confluent preadipocytes. Finally, exposure of primary cultured preadipocytes from
the subcutaneous area to leptin (10 nM) resulted in an increased proliferation ([3H]thymidine incorporation and cell
counting) and differentiation (glycerol-3-phosphate dehydrogenase
activity and mRNA levels of lipoprotein lipase, peroxisome
proliferator-activated receptor-
2, and c-fos). Altogether, these
results indicate that, in vitro at least, leptin through its functional
receptors exerts a proadipogenic action in subcutaneous preadipocytes.
leptin receptors; mitogen-activated protein kinase; adipogenesis
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