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1 Institut National de la Santé et de la Recherche Médicale (INSERM), Unité 467, Faculté de Médecine Necker-Enfants Malades, 75015 Paris; 2 INSERM, Unité 410, Institut Fédératif de Recherche Xavier Bichat, 75018 Paris; 3 INSERM, Unité 468, Hôpital Henri Mondor, 94010 Créteil; and 4 Centre National de la Recherche Scientifique, Unité Propre de Recherche 1524, Hôpital Saint Vincent de Paul, 75674 Paris, France
The ubiquitous ClC-2 Cl
channel is thought to contribute to epithelial Cl
secretion, but the distribution of the ClC-2 protein in human epithelia
has not been investigated. We have studied the distribution of ClC-2 in
adult human and rat intestine and airways by immunoblotting and
confocal microscopy. In the rat, ClC-2 was present in the lateral
membranes of villus enterocytes and was predominant at the basolateral
membranes of luminal colon enterocytes. The expression pattern of ClC-2
in the human intestine differed significantly, because ClC-2 was mainly
detected in a supranuclear compartment of colon cells. We found
significant expression of ClC-2 at the apex of ciliated cells in both
rat and human airways. These results show that the distribution of
ClC-2 in airways is consistent with participation of ClC-2 channels in
Cl secretion and indicate that extrapolation of results
from studies of ClC-2 function in rat intestine to human intestine is
not straightforward.
small intestine; colon; ciliated cells; immunohistochemistry; antibody
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