Am J Physiol Cell Physiol Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 282: C754-C761, 2002. First published November 27, 2001; doi:10.1152/ajpcell.00335.2001
0363-6143/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/4/C754    most recent
00335.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (54)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ginnan, R.
Right arrow Articles by Singer, H. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ginnan, R.
Right arrow Articles by Singer, H. A.
Vol. 282, Issue 4, C754-C761, April 2002

CaM kinase II-dependent activation of tyrosine kinases and ERK1/2 in vascular smooth muscle

Roman Ginnan and Harold A. Singer

Center for Cardiovascular Sciences, Albany Medical College, Albany, New York 12208

In vascular smooth muscle (VSM) and many other cells, G protein receptor-coupled activation of mitogen-activated protein kinases has been linked, in part, to increases in free intracellular Ca2+. Previously, we demonstrated that ionomycin-, angiotensin II-, and thrombin-induced activation of extracellular signal-regulated kinase (ERK)1/2 in VSM cells was attenuated by pretreatment with KN-93, a selective inhibitor of the multifunctional Ca2+/calmodulin-dependent protein kinase (CaM kinase II). In the present study, we show that the Ca2+-dependent pathway leading to activation of ERK1/2 is preceded by nonreceptor proline-rich tyrosine kinase (PYK2) activation and epidermal growth factor (EGF) receptor tyrosine phosphorylation and is attenuated by inhibitors of src family kinases or the EGF receptor tyrosine kinase. Furthermore, we demonstrate that pretreatment with KN-93 or a CaM kinase II inhibitor peptide inhibits Ca2+-dependent PYK2 activation and EGF receptor tyrosine phosphorylation in response to ionomycin, ATP, and platelet-derived growth factor but has no effect on phorbol 12,13-dibutyrate- or EGF-induced responses. The results implicate CaM kinase II as an intermediate in the Ca2+/calmodulin-dependent activation of PYK2.

calcium; calcium/calmodulin-dependent protein kinase; proline-rich tyrosine kinase; extracellular signal-regulated kinase 1/2; epidermal growth factor receptor transactivation; src; vascular smooth muscle cells; KN-93; autoinhibitory peptide


This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
R. Cheung, M. Malik, V. Ravyn, B. Tomkowicz, A. Ptasznik, and R. G. Collman
An arrestin-dependent multi-kinase signaling complex mediates MIP-1{beta}/CCL4 signaling and chemotaxis of primary human macrophages
J. Leukoc. Biol., October 1, 2009; 86(4): 833 - 845.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. Z. Mercure, R. Ginnan, and H. A. Singer
CaM kinase II{delta}2-dependent regulation of vascular smooth muscle cell polarization and migration
Am J Physiol Cell Physiol, June 1, 2008; 294(6): C1465 - C1475.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
J. Pang, C. Yan, K. Natarajan, M. E. Cavet, M. P. Massett, G. Yin, and B. C. Berk
GIT1 Mediates HDAC5 Activation by Angiotensin II in Vascular Smooth Muscle Cells
Arterioscler Thromb Vasc Biol, May 1, 2008; 28(5): 892 - 898.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
S. J. House and H. A. Singer
CaMKII-{delta} Isoform Regulation of Neointima Formation After Vascular Injury
Arterioscler Thromb Vasc Biol, March 1, 2008; 28(3): 441 - 447.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
Y. J. Lee and H. J. Han
Albumin-stimulated DNA synthesis is mediated by Ca2+/PKC as well as EGF receptor-dependent p44/42 MAPK and NF-{kappa}B signal pathways in renal proximal tubule cells
Am J Physiol Renal Physiol, March 1, 2008; 294(3): F534 - F541.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. M. Houston, H. H. C. Lee, A. M. Hosie, S. J. Moss, and T. G. Smart
Identification of the Sites for CaMK-II-dependent Phosphorylation of GABAA Receptors
J. Biol. Chem., June 15, 2007; 282(24): 17855 - 17865.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. J. House, R. G. Ginnan, S. E. Armstrong, and H. A. Singer
Calcium/calmodulin-dependent protein kinase II-{delta} isoform regulation of vascular smooth muscle cell proliferation
Am J Physiol Cell Physiol, June 1, 2007; 292(6): C2276 - C2287.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. J. Jones, D. Jourd'heuil, J. C. Salerno, S. M. E. Smith, and H. A. Singer
iNOS regulation by calcium/calmodulin-dependent protein kinase II in vascular smooth muscle
Am J Physiol Heart Circ Physiol, June 1, 2007; 292(6): H2634 - H2642.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
N. V. Bogatcheva, P. Wang, A. A. Birukova, A. D. Verin, and J. G. N. Garcia
Mechanism of fluoride-induced MAP kinase activation in pulmonary artery endothelial cells
Am J Physiol Lung Cell Mol Physiol, June 1, 2006; 290(6): L1139 - L1145.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. Ginnan, B. J. Guikema, H. A. Singer, and D. Jourd'heuil
PKC-{delta} mediates activation of ERK1/2 and induction of iNOS by IL-1beta in vascular smooth muscle cells
Am J Physiol Cell Physiol, June 1, 2006; 290(6): C1583 - C1591.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
D. C. Wright, P. C. Geiger, D.-H. Han, and J. O. Holloszy
Are tyrosine kinases involved in mediating contraction-stimulated muscle glucose transport?
Am J Physiol Endocrinol Metab, January 1, 2006; 290(1): E123 - E128.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
T. L. Herrmann, C. T. Morita, K. Lee, and D. J. Kusner
Calmodulin kinase II regulates the maturation and antigen presentation of human dendritic cells
J. Leukoc. Biol., December 1, 2005; 78(6): 1397 - 1407.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
K. K. Lu, S. E. Armstrong, R. Ginnan, and H. A. Singer
Adhesion-dependent activation of CaMKII and regulation of ERK activation in vascular smooth muscle
Am J Physiol Cell Physiol, November 1, 2005; 289(5): C1343 - C1350.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
W. A. Marganski, S. S. Gangopadhyay, H.-D. Je, C. Gallant, and K. G. Morgan
Targeting of a Novel Ca+2/Calmodulin-Dependent Protein Kinase II Is Essential for Extracellular Signal-Regulated Kinase-Mediated Signaling in Differentiated Smooth Muscle Cells
Circ. Res., September 16, 2005; 97(6): 541 - 549.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. Ginnan and H. A. Singer
PKC-{delta}-dependent pathways contribute to PDGF-stimulated ERK1/2 activation in vascular smooth muscle
Am J Physiol Cell Physiol, June 1, 2005; 288(6): C1193 - C1201.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
G. P. Sergeant, S. Ohya, J. A. Reihill, B. A. Perrino, G. C. Amberg, Y. Imaizumi, B. Horowitz, K. M. Sanders, and S. D. Koh
Regulation of Kv4.3 currents by Ca2+/calmodulin-dependent protein kinase II
Am J Physiol Cell Physiol, February 1, 2005; 288(2): C304 - C313.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Azriel-Tamir, H. Sharir, B. Schwartz, and M. Hershfinkel
Extracellular Zinc Triggers ERK-dependent Activation of Na+/H+ Exchange in Colonocytes Mediated by the Zinc-sensing Receptor
J. Biol. Chem., December 10, 2004; 279(50): 51804 - 51816.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. Ginnan, P. J. Pfleiderer, K. Pumiglia, and H. A. Singer
PKC-{delta} and CaMKII-{delta}2 mediate ATP-dependent activation of ERK1/2 in vascular smooth muscle
Am J Physiol Cell Physiol, June 1, 2004; 286(6): C1281 - C1289.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
G. Yin, J. Haendeler, C. Yan, and B. C. Berk
GIT1 Functions as a Scaffold for MEK1-Extracellular Signal-Regulated Kinase 1 and 2 Activation by Angiotensin II and Epidermal Growth Factor
Mol. Cell. Biol., January 15, 2004; 24(2): 875 - 885.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. Bobe, X. Yin, M.-C. Roussanne, O. Stepien, E. Polidano, C. Faverdin, and P. Marche
Evidence for ERK1/2 activation by thrombin that is independent of EGFR transactivation
Am J Physiol Heart Circ Physiol, July 11, 2003; 285(2): H745 - H754.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online