Am J Physiol Cell Physiol AJP: Advances in Physiology Education
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 282: C617-C624, 2002. First published October 31, 2001; doi:10.1152/ajpcell.00271.2001
0363-6143/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/3/C617    most recent
00271.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (52)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stamenovic, D.
Right arrow Articles by Wang, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stamenovic, D.
Right arrow Articles by Wang, N.
Vol. 282, Issue 3, C617-C624, March 2002

Cell prestress. II. Contribution of microtubules

Dimitrije Stamenovic'1, Srboljub M. Mijailovich2, Iva Marija Tolic'-Nørrelykke2,3, Jianxin Chen2, and Ning Wang2

1 Department of Biomedical Engineering, Boston University, Boston 02215; 2 Physiology Program, Department of Environmental Health, Harvard School of Public Health, Boston, Massachusetts 02115; and 3 Rugjer Boskovic' Institute, 10001 Zagreb, Croatia

The tensegrity model hypothesizes that cytoskeleton-based microtubules (MTs) carry compression as they balance a portion of cell contractile stress. To test this hypothesis, we used traction force microscopy to measure traction at the interface of adhering human airway smooth muscle cells and a flexible polyacrylamide gel substrate. The prediction is that if MTs balance a portion of contractile stress, then, upon their disruption, the portion of stress balanced by MTs would shift to the substrate, thereby causing an increase in traction. Measurements were done first in maximally activated cells (10 µM histamine) and then again after MTs had been disrupted (1 µM colchicine). We found that after disruption of MTs, traction increased on average by ~13%. Because in activated cells colchicine induced neither an increase in intracellular Ca2+ nor an increase in myosin light chain phosphorylation as shown previously, we concluded that the observed increase in traction was a result of load shift from MTs to the substrate. In addition, energy stored in the flexible substrate was calculated as work done by traction on the deformation of the substrate. This result was then utilized in an energetic analysis. We assumed that cytoskeleton-based MTs are slender elastic rods supported laterally by intermediate filaments and that MTs buckle as the cell contracts. Using the post-buckling equilibrium theory of Euler struts, we found that energy stored during buckling of MTs was quantitatively consistent with the measured increase in substrate energy after disruption of MTs. This is further evidence supporting the idea that MTs are intracellular compression-bearing elements.

cytoskeleton; compression; energy; traction; tensegrity


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
A. M. Goldyn, B. A. Rioja, J. P. Spatz, C. Ballestrem, and R. Kemkemer
Force-induced cell polarisation is linked to RhoA-driven microtubule-independent focal-adhesion sliding
J. Cell Sci., October 15, 2009; 122(20): 3644 - 3651.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
R. C. Geiger, C. D. Kaufman, A. P. Lam, G. R. S. Budinger, and D. A. Dean
Tubulin Acetylation and Histone Deacetylase 6 Activity in the Lung under Cyclic Load
Am. J. Respir. Cell Mol. Biol., January 1, 2009; 40(1): 76 - 82.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
K. Nagayama and T. Matsumoto
Contribution of actin filaments and microtubules to quasi-in situ tensile properties and internal force balance of cultured smooth muscle cells on a substrate
Am J Physiol Cell Physiol, December 1, 2008; 295(6): C1569 - C1578.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C. S. Chen
Mechanotransduction - a field pulling together?
J. Cell Sci., October 15, 2008; 121(20): 3285 - 3292.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
C. P. Brangwynne, F. C. MacKintosh, S. Kumar, N. A. Geisse, J. Talbot, L. Mahadevan, K. K. Parker, D. E. Ingber, and D. A. Weitz
Microtubules can bear enhanced compressive loads in living cells because of lateral reinforcement
J. Cell Biol., June 5, 2006; 173(5): 733 - 741.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Ito, A. Majumdar, H. Kume, K. Shimokata, K. Naruse, K. R. Lutchen, D. Stamenovic, and B. Suki
Viscoelastic and dynamic nonlinear properties of airway smooth muscle tissue: roles of mechanical force and the cytoskeleton
Am J Physiol Lung Cell Mol Physiol, June 1, 2006; 290(6): L1227 - L1237.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
P. C. Georges and P. A. Janmey
Cell type-specific response to growth on soft materials
J Appl Physiol, April 1, 2005; 98(4): 1547 - 1553.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
B. P. Helmke
Molecular Control of Cytoskeletal Mechanics by Hemodynamic Forces
Physiology, February 1, 2005; 20(1): 43 - 53.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
S. P. Evanko, W. T. Parks, and T. N. Wight
Intracellular Hyaluronan in Arterial Smooth Muscle Cells: Association with Microtubules, RHAMM, and the Mitotic Spindle
J. Histochem. Cytochem., December 1, 2004; 52(12): 1525 - 1536.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. Stamenovic, B. Suki, B. Fabry, N. Wang, J. J. Fredberg, and J. E. Buy
Rheology of airway smooth muscle cells is associated with cytoskeletal contractile stress
J Appl Physiol, May 1, 2004; 96(5): 1600 - 1605.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
S. S. An, B. Fabry, M. Mellema, P. Bursac, W. T. Gerthoffer, U. S. Kayyali, M. Gaestel, S. A. Shore, and J. J. Fredberg
Role of heat shock protein 27 in cytoskeletal remodeling of the airway smooth muscle cell
J Appl Physiol, May 1, 2004; 96(5): 1701 - 1713.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. Hu, J. Chen, B. Fabry, Y. Numaguchi, A. Gouldstone, D. E. Ingber, J. J. Fredberg, J. P. Butler, and N. Wang
Intracellular stress tomography reveals stress focusing and structural anisotropy in cytoskeleton of living cells
Am J Physiol Cell Physiol, November 1, 2003; 285(5): C1082 - C1090.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
S. J. Gunst and J. J. Fredberg
The first three minutes: smooth muscle contraction, cytoskeletal events, and soft glasses
J Appl Physiol, July 1, 2003; 95(1): 413 - 425.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. E. Ingber
Tensegrity I. Cell structure and hierarchical systems biology
J. Cell Sci., April 1, 2003; 116(7): 1157 - 1173.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
N. Wang, I. M. Tolic-Norrelykke, J. Chen, S. M. Mijailovich, J. P. Butler, J. J. Fredberg, and D. Stamenovic
Cell prestress. I. Stiffness and prestress are closely associated in adherent contractile cells
Am J Physiol Cell Physiol, March 1, 2002; 282(3): C606 - C616.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. P. Butler, I. M. Tolic-Norrelykke, B. Fabry, and J. J. Fredberg
Traction fields, moments, and strain energy that cells exert on their surroundings
Am J Physiol Cell Physiol, March 1, 2002; 282(3): C595 - C605.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online