Am J Physiol Cell Physiol Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 282: C606-C616, 2002. First published October 31, 2001; doi:10.1152/ajpcell.00269.2001
0363-6143/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/3/C606    most recent
00269.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (132)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, N.
Right arrow Articles by Stamenovic, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, N.
Right arrow Articles by Stamenovic, D.
Vol. 282, Issue 3, C606-C616, March 2002

Cell prestress. I. Stiffness and prestress are closely associated in adherent contractile cells

Ning Wang1, Iva Marija Tolic'-Nørrelykke1,3, Jianxin Chen1, Srboljub M. Mijailovich1, James P. Butler1, Jeffrey J. Fredberg1, and Dimitrije Stamenovic'2

1 Physiology Program, Department of Environmental Health, Harvard School of Public Health, Boston 02115; 2 Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215; and 3 Rugjer Boskovic' Institute, 10001 Zagreb, Croatia

The tensegrity hypothesis holds that the cytoskeleton is a structure whose shape is stabilized predominantly by the tensile stresses borne by filamentous structures. Accordingly, cell stiffness must increase in proportion with the level of the tensile stress, which is called the prestress. Here we have tested that prediction in adherent human airway smooth muscle (HASM) cells. Traction microscopy was used to measure the distribution of contractile stresses arising at the interface between each cell and its substrate; this distribution is called the traction field. Because the traction field must be balanced by tensile stresses within the cell body, the prestress could be computed. Cell stiffness (G) was measured by oscillatory magnetic twisting cytometry. As the contractile state of the cell was modulated with graded concentrations of relaxing or contracting agonists (isoproterenol or histamine, respectively), the mean prestress (<A><AC>p</AC><AC>&cjs1171;</AC></A>t) ranged from 350 to 1,900 Pa. Over that range, cell stiffness increased linearly with the prestress: G (Pa) = 0.18<A><AC>p</AC><AC>&cjs1171;</AC></A>t + 92. While this association does not necessarily preclude other interpretations, it is the hallmark of systems that secure shape stability mainly through the prestress. Regardless of mechanism, these data establish a strong association between stiffness of HASM cells and the level of tensile stress within the cytoskeleton.

tensegrity; mechanical stress; traction; cytoskeleton; actin microfilaments


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
E. H. Zhou, X. Trepat, C. Y. Park, G. Lenormand, M. N. Oliver, S. M. Mijailovich, C. Hardin, D. A. Weitz, J. P. Butler, and J. J. Fredberg
Universal behavior of the osmotically compressed cell and its analogy to the colloidal glass transition
PNAS, June 30, 2009; 106(26): 10632 - 10637.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. Na, F. Chowdhury, B. Tay, M. Ouyang, M. Gregor, Y. Wang, G. Wiche, and N. Wang
Plectin contributes to mechanical properties of living cells
Am J Physiol Cell Physiol, April 1, 2009; 296(4): C868 - C877.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
K. Nagayama and T. Matsumoto
Contribution of actin filaments and microtubules to quasi-in situ tensile properties and internal force balance of cultured smooth muscle cells on a substrate
Am J Physiol Cell Physiol, December 1, 2008; 295(6): C1569 - C1578.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. J. Engler, C. Carag-Krieger, C. P. Johnson, M. Raab, H.-Y. Tang, D. W. Speicher, J. W. Sanger, J. M. Sanger, and D. E. Discher
Embryonic cardiomyocytes beat best on a matrix with heart-like elasticity: scar-like rigidity inhibits beating
J. Cell Sci., November 15, 2008; 121(22): 3794 - 3802.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
N. J. Fairbank, S. C. Connolly, J. D. MacKinnon, K. Wehry, L. Deng, and G. N. Maksym
Airway smooth muscle cell tone amplifies contractile function in the presence of chronic cyclic strain
Am J Physiol Lung Cell Mol Physiol, September 1, 2008; 295(3): L479 - L488.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
K. A. Addae-Mensah and J. P. Wikswo
Measurement Techniques for Cellular Biomechanics In Vitro
Experimental Biology and Medicine, July 1, 2008; 233(7): 792 - 809.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. R. Klinger, J. D. Murray, B. Casserly, D. F. Alvarez, J. A. King, S. S. An, G. Choudhary, A. N. Owusu-Sarfo, R. Warburton, and E. O. Harrington
Rottlerin causes pulmonary edema in vivo: a possible role for PKC{delta}
J Appl Physiol, December 1, 2007; 103(6): 2084 - 2094.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
C. P. Johnson, H.-Y. Tang, C. Carag, D. W. Speicher, and D. E. Discher
Forced Unfolding of Proteins Within Cells
Science, August 3, 2007; 317(5838): 663 - 666.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
N. Gavara, R. Sunyer, P. Roca-Cusachs, R. Farre, M. Rotger, and D. Navajas
Thrombin-induced contraction in alveolar epithelial cells probed by traction microscopy
J Appl Physiol, August 1, 2006; 101(2): 512 - 520.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
F. J. Byfield, S. Tikku, G. H. Rothblat, K. J. Gooch, and I. Levitan
OxLDL increases endothelial stiffness, force generation, and network formation
J. Lipid Res., April 1, 2006; 47(4): 715 - 723.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
B. D. Matthews, D. R. Overby, R. Mannix, and D. E. Ingber
Cellular adaptation to mechanical stress: role of integrins, Rho, cytoskeletal tension and mechanosensitive ion channels
J. Cell Sci., February 1, 2006; 119(3): 508 - 518.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. S. An, C. M. Pennella, A. Gonnabathula, J. Chen, N. Wang, M. Gaestel, P. M. Hassoun, J. J. Fredberg, and U. S. Kayyali
Hypoxia alters biophysical properties of endothelial cells via p38 MAPK- and Rho kinase-dependent pathways
Am J Physiol Cell Physiol, September 1, 2005; 289(3): C521 - C530.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
L. Deng, N. J. Fairbank, D. J. Cole, J. J. Fredberg, and G. N. Maksym
Airway smooth muscle tone modulates mechanically induced cytoskeletal stiffening and remodeling
J Appl Physiol, August 1, 2005; 99(2): 634 - 641.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. A. Griffin, H. Feng, M. Tewari, P. Acosta, M. Kawana, H. L. Sweeney, and D. E. Discher
{gamma}-Sarcoglycan deficiency increases cell contractility, apoptosis and MAPK pathway activation but does not affect adhesion
J. Cell Sci., April 1, 2005; 118(7): 1405 - 1416.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
B. P. Helmke
Molecular Control of Cytoskeletal Mechanics by Hemodynamic Forces
Physiology, February 1, 2005; 20(1): 43 - 53.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. A. Griffin, S. Sen, H. L. Sweeney, and D. E. Discher
Adhesion-contractile balance in myocyte differentiation
J. Cell Sci., November 15, 2004; 117(24): 5855 - 5863.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. Hu, L. Eberhard, J. Chen, J. C. Love, J. P. Butler, J. J. Fredberg, G. M. Whitesides, and N. Wang
Mechanical anisotropy of adherent cells probed by a three-dimensional magnetic twisting device
Am J Physiol Cell Physiol, November 1, 2004; 287(5): C1184 - C1191.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
A. J. Engler, M. A. Griffin, S. Sen, C. G. Bonnemann, H. L. Sweeney, and D. E. Discher
Myotubes differentiate optimally on substrates with tissue-like stiffness: pathological implications for soft or stiff microenvironments
J. Cell Biol., September 13, 2004; 166(6): 877 - 887.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. Puig-de-Morales, E. Millet, B. Fabry, D. Navajas, N. Wang, J. P. Butler, and J. J. Fredberg
Cytoskeletal mechanics in adherent human airway smooth muscle cells: probe specificity and scaling of protein-protein dynamics
Am J Physiol Cell Physiol, September 1, 2004; 287(3): C643 - C654.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
Mechanisms and Limits of Induced Postnatal Lung Growth
Am. J. Respir. Crit. Care Med., August 1, 2004; 170(3): 319 - 343.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. Deng, N. J. Fairbank, B. Fabry, P. G. Smith, and G. N. Maksym
Localized mechanical stress induces time-dependent actin cytoskeletal remodeling and stiffening in cultured airway smooth muscle cells
Am J Physiol Cell Physiol, August 1, 2004; 287(2): C440 - C448.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. B. Moy, K. Blackwell, N. Wang, K. Haxhinasto, M. K. Kasiske, J. Bodmer, G. Reyes, and A. English
Phorbol ester-mediated pulmonary artery endothelial barrier dysfunction through regulation of actin cytoskeletal mechanics
Am J Physiol Lung Cell Mol Physiol, July 1, 2004; 287(1): L153 - L167.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. Stamenovic, B. Suki, B. Fabry, N. Wang, J. J. Fredberg, and J. E. Buy
Rheology of airway smooth muscle cells is associated with cytoskeletal contractile stress
J Appl Physiol, May 1, 2004; 96(5): 1600 - 1605.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
T. R. Polte, G. S. Eichler, N. Wang, and D. E. Ingber
Extracellular matrix controls myosin light chain phosphorylation and cell contractility through modulation of cell shape and cytoskeletal prestress
Am J Physiol Cell Physiol, March 1, 2004; 286(3): C518 - C528.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. Hu, J. Chen, B. Fabry, Y. Numaguchi, A. Gouldstone, D. E. Ingber, J. J. Fredberg, J. P. Butler, and N. Wang
Intracellular stress tomography reveals stress focusing and structural anisotropy in cytoskeleton of living cells
Am J Physiol Cell Physiol, November 1, 2003; 285(5): C1082 - C1090.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
P. G. Smith, L. Deng, J. J. Fredberg, and G. N. Maksym
Mechanical strain increases cell stiffness through cytoskeletal filament reorganization
Am J Physiol Lung Cell Mol Physiol, August 1, 2003; 285(2): L456 - L463.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
S. J. Gunst and J. J. Fredberg
The first three minutes: smooth muscle contraction, cytoskeletal events, and soft glasses
J Appl Physiol, July 1, 2003; 95(1): 413 - 425.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. Y. Boateng, T. J. Hartman, N. Ahluwalia, H. Vidula, T. A. Desai, and B. Russell
Inhibition of fibroblast proliferation in cardiac myocyte cultures by surface microtopography
Am J Physiol Cell Physiol, July 1, 2003; 285(1): C171 - C182.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. E. Ingber
Tensegrity I. Cell structure and hierarchical systems biology
J. Cell Sci., April 1, 2003; 116(7): 1157 - 1173.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
I. M. Tolic-Norrelykke, J. P. Butler, J. Chen, and N. Wang
Spatial and temporal traction response in human airway smooth muscle cells
Am J Physiol Cell Physiol, October 1, 2002; 283(4): C1254 - C1266.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. S. An, R. E. Laudadio, J. Lai, R. A. Rogers, and J. J. Fredberg
Stiffness changes in cultured airway smooth muscle cells
Am J Physiol Cell Physiol, September 1, 2002; 283(3): C792 - C801.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
A. Katsumi, J. Milanini, W. B. Kiosses, M. A. del Pozo, R. Kaunas, S. Chien, K. M. Hahn, and M. A. Schwartz
Effects of cell tension on the small GTPase Rac
J. Cell Biol., July 8, 2002; 158(1): 153 - 164.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. P. Butler, I. M. Tolic-Norrelykke, B. Fabry, and J. J. Fredberg
Traction fields, moments, and strain energy that cells exert on their surroundings
Am J Physiol Cell Physiol, March 1, 2002; 282(3): C595 - C605.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D. Stamenovic, S. M. Mijailovich, I. M. Tolic-Norrelykke, J. Chen, and N. Wang
Cell prestress. II. Contribution of microtubules
Am J Physiol Cell Physiol, March 1, 2002; 282(3): C617 - C624.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online